Proteomic profiling: A novel method for differential diagnosis?

Sven Gläsker, Russell R. Lonser, Hiroaki Okamoto, Jie Li, Howard Jaffee, Edward H. Oldfield, Zhengping Zhuang, Alexander Vortmeyer

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Tumors with identical phenotype can have markedly different biologic behavior. The differentiation between hemangioblastoma, a benign vascular brain tumor, and renal cell carcinoma (RCC), a malignant tumor that can metastasize to the brain, is a well-known pathological quandary. We report a 59-year-old female with von Hippel-Lindau disease and known RCC. She presented with a cystic cerebellar tumor that had typical MRI appearance of a hemangioblastoma. Progressive symptoms led to surgical intervention. The histology included features consistent with either hemangioblastoma with epitheloid phenotype or atypical RCC metastasis mimicking hemangioblastoma. The discrepancy between the benign clinical course and the histologic phenotype could not be clearly resolved using conventional clinical techniques. We used proteomic profiling to clarify this diagnostic dilemma. We compared the proteomic expression pattern of the unknown lesion with the patterns of three typical hemangioblastomas and three typical RCCs by using selective tissue microdissection combined with two-dimensional proteomic profiling and protein sequencing. The results clearly indicated a protein profile consistent with RCC. Proteomics may complement pathological evaluation and characterization of tumors in the future. Furthermore, these results show that metastasis of malignant RCC is not necessarily equivalent to aggressive growth patterns.

Original languageEnglish (US)
Pages (from-to)343-345
Number of pages3
JournalCancer Biology and Therapy
Volume6
Issue number3
DOIs
StatePublished - Jan 1 2007
Externally publishedYes

Fingerprint

Hemangioblastoma
Renal Cell Carcinoma
Proteomics
Differential Diagnosis
Phenotype
Cerebellar Neoplasms
Neoplasm Metastasis
von Hippel-Lindau Disease
Neoplasms
Microdissection
Protein Sequence Analysis
Brain Neoplasms
Blood Vessels
Histology
Brain
Growth
Proteins

Keywords

  • Hemangioblastoma
  • Proteomics
  • Renal carcinoma
  • Von Hippel Lindau disease

ASJC Scopus subject areas

  • Molecular Medicine
  • Oncology
  • Pharmacology
  • Cancer Research

Cite this

Gläsker, S., Lonser, R. R., Okamoto, H., Li, J., Jaffee, H., Oldfield, E. H., ... Vortmeyer, A. (2007). Proteomic profiling: A novel method for differential diagnosis? Cancer Biology and Therapy, 6(3), 343-345. https://doi.org/10.4161/cbt.6.3.3673

Proteomic profiling : A novel method for differential diagnosis? / Gläsker, Sven; Lonser, Russell R.; Okamoto, Hiroaki; Li, Jie; Jaffee, Howard; Oldfield, Edward H.; Zhuang, Zhengping; Vortmeyer, Alexander.

In: Cancer Biology and Therapy, Vol. 6, No. 3, 01.01.2007, p. 343-345.

Research output: Contribution to journalArticle

Gläsker, S, Lonser, RR, Okamoto, H, Li, J, Jaffee, H, Oldfield, EH, Zhuang, Z & Vortmeyer, A 2007, 'Proteomic profiling: A novel method for differential diagnosis?', Cancer Biology and Therapy, vol. 6, no. 3, pp. 343-345. https://doi.org/10.4161/cbt.6.3.3673
Gläsker S, Lonser RR, Okamoto H, Li J, Jaffee H, Oldfield EH et al. Proteomic profiling: A novel method for differential diagnosis? Cancer Biology and Therapy. 2007 Jan 1;6(3):343-345. https://doi.org/10.4161/cbt.6.3.3673
Gläsker, Sven ; Lonser, Russell R. ; Okamoto, Hiroaki ; Li, Jie ; Jaffee, Howard ; Oldfield, Edward H. ; Zhuang, Zhengping ; Vortmeyer, Alexander. / Proteomic profiling : A novel method for differential diagnosis?. In: Cancer Biology and Therapy. 2007 ; Vol. 6, No. 3. pp. 343-345.
@article{52de3368b1a04911a36e230b11afebeb,
title = "Proteomic profiling: A novel method for differential diagnosis?",
abstract = "Tumors with identical phenotype can have markedly different biologic behavior. The differentiation between hemangioblastoma, a benign vascular brain tumor, and renal cell carcinoma (RCC), a malignant tumor that can metastasize to the brain, is a well-known pathological quandary. We report a 59-year-old female with von Hippel-Lindau disease and known RCC. She presented with a cystic cerebellar tumor that had typical MRI appearance of a hemangioblastoma. Progressive symptoms led to surgical intervention. The histology included features consistent with either hemangioblastoma with epitheloid phenotype or atypical RCC metastasis mimicking hemangioblastoma. The discrepancy between the benign clinical course and the histologic phenotype could not be clearly resolved using conventional clinical techniques. We used proteomic profiling to clarify this diagnostic dilemma. We compared the proteomic expression pattern of the unknown lesion with the patterns of three typical hemangioblastomas and three typical RCCs by using selective tissue microdissection combined with two-dimensional proteomic profiling and protein sequencing. The results clearly indicated a protein profile consistent with RCC. Proteomics may complement pathological evaluation and characterization of tumors in the future. Furthermore, these results show that metastasis of malignant RCC is not necessarily equivalent to aggressive growth patterns.",
keywords = "Hemangioblastoma, Proteomics, Renal carcinoma, Von Hippel Lindau disease",
author = "Sven Gl{\"a}sker and Lonser, {Russell R.} and Hiroaki Okamoto and Jie Li and Howard Jaffee and Oldfield, {Edward H.} and Zhengping Zhuang and Alexander Vortmeyer",
year = "2007",
month = "1",
day = "1",
doi = "10.4161/cbt.6.3.3673",
language = "English (US)",
volume = "6",
pages = "343--345",
journal = "Cancer Biology and Therapy",
issn = "1538-4047",
publisher = "Landes Bioscience",
number = "3",

}

TY - JOUR

T1 - Proteomic profiling

T2 - A novel method for differential diagnosis?

AU - Gläsker, Sven

AU - Lonser, Russell R.

AU - Okamoto, Hiroaki

AU - Li, Jie

AU - Jaffee, Howard

AU - Oldfield, Edward H.

AU - Zhuang, Zhengping

AU - Vortmeyer, Alexander

PY - 2007/1/1

Y1 - 2007/1/1

N2 - Tumors with identical phenotype can have markedly different biologic behavior. The differentiation between hemangioblastoma, a benign vascular brain tumor, and renal cell carcinoma (RCC), a malignant tumor that can metastasize to the brain, is a well-known pathological quandary. We report a 59-year-old female with von Hippel-Lindau disease and known RCC. She presented with a cystic cerebellar tumor that had typical MRI appearance of a hemangioblastoma. Progressive symptoms led to surgical intervention. The histology included features consistent with either hemangioblastoma with epitheloid phenotype or atypical RCC metastasis mimicking hemangioblastoma. The discrepancy between the benign clinical course and the histologic phenotype could not be clearly resolved using conventional clinical techniques. We used proteomic profiling to clarify this diagnostic dilemma. We compared the proteomic expression pattern of the unknown lesion with the patterns of three typical hemangioblastomas and three typical RCCs by using selective tissue microdissection combined with two-dimensional proteomic profiling and protein sequencing. The results clearly indicated a protein profile consistent with RCC. Proteomics may complement pathological evaluation and characterization of tumors in the future. Furthermore, these results show that metastasis of malignant RCC is not necessarily equivalent to aggressive growth patterns.

AB - Tumors with identical phenotype can have markedly different biologic behavior. The differentiation between hemangioblastoma, a benign vascular brain tumor, and renal cell carcinoma (RCC), a malignant tumor that can metastasize to the brain, is a well-known pathological quandary. We report a 59-year-old female with von Hippel-Lindau disease and known RCC. She presented with a cystic cerebellar tumor that had typical MRI appearance of a hemangioblastoma. Progressive symptoms led to surgical intervention. The histology included features consistent with either hemangioblastoma with epitheloid phenotype or atypical RCC metastasis mimicking hemangioblastoma. The discrepancy between the benign clinical course and the histologic phenotype could not be clearly resolved using conventional clinical techniques. We used proteomic profiling to clarify this diagnostic dilemma. We compared the proteomic expression pattern of the unknown lesion with the patterns of three typical hemangioblastomas and three typical RCCs by using selective tissue microdissection combined with two-dimensional proteomic profiling and protein sequencing. The results clearly indicated a protein profile consistent with RCC. Proteomics may complement pathological evaluation and characterization of tumors in the future. Furthermore, these results show that metastasis of malignant RCC is not necessarily equivalent to aggressive growth patterns.

KW - Hemangioblastoma

KW - Proteomics

KW - Renal carcinoma

KW - Von Hippel Lindau disease

UR - http://www.scopus.com/inward/record.url?scp=34548225428&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548225428&partnerID=8YFLogxK

U2 - 10.4161/cbt.6.3.3673

DO - 10.4161/cbt.6.3.3673

M3 - Article

C2 - 17471020

AN - SCOPUS:34548225428

VL - 6

SP - 343

EP - 345

JO - Cancer Biology and Therapy

JF - Cancer Biology and Therapy

SN - 1538-4047

IS - 3

ER -