Proteomic profiling of human sera for discovery of potential biomarkers to monitor abstinence from alcohol abuse

Xianyin Lai, Suthat Liangpunsakul, Kaigang Li, Frank A. Witzmann

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Although numerous biomarkers or biomarker candidates have been discovered to detect levels of drinking and intervals of time after last drinking episode, only a few biomarkers have been applied to monitor abstinence in a longer interval (≥6 wks) from alcohol abuse. Considering sample sources, sensitivity, and specificity, new biomarkers from blood with better accuracy are needed. To address this, serum proteomic profiles were compared between pre- and post- treatment samples from subjects seeking treatment for alcohol abuse and dependence in an intensive 6wk daily outpatient program using high-abundance plasma protein immunodepletion and LC-MS/MS techniques. Protein identification, quantification, candidate biomarker selection, and prioritization analyses were carried out. Among the 246 quantified serum proteins, abundance of 13 and 45 proteins in female and male subjects were significantly changed (p ≤ 0.05), respectively. Of these biomarker candidate proteins, 2 (female) and 8 (male) proteins were listed in category 1, with high area under the receiver operating characteristic curve, sensitivity, specificity, and fold change. In summary, several new biomarker candidates have been identified to monitor abstinence from alcohol abuse.

Original languageEnglish (US)
Pages (from-to)556-563
Number of pages8
JournalELECTROPHORESIS
Volume36
Issue number4
DOIs
StatePublished - Feb 1 2015

Keywords

  • Alcohol Abuse
  • Biomarker
  • MS/MS
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

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