Proteomics analysis of starved cells revealed Annexin A1 as an important regulator of autophagic degradation

Jeong Han Kang, Min Li, Xi Chen, Xiao-Ming Yin

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Macroautophagy is involved in the bulk degradation of long-lived cytosolic proteins and subcellular organelles, which is important for the survival of cells during starvation. To identify potential players of the autophagy process, we subjected HCT116 cells cultured in complete medium and in Earle's balanced salt solution to proteomics analysis. In approximately 1500 protein spots detected, we characterized 52 unique proteins, whose expression levels were significantly changed following starvation. Notably, we found that Annexin A1 was significantly upregulated following starvation at both mRNA and protein levels. Inhibition of Annexin A1 expression with specific siRNA did not alter starvation-induced autophagy as measured by the level of lipidated LC3, but significantly reversed autophagy degradation as measured by the level of p62/SQSTM 1. Thus Annexin A1 seemed to be positively upregulated during starvation to promote autophagic degradation. Overall, the data presented in this study established a expression profile of the proteome in starved cells, which allowed the identification of proteins with potential significance in starvation-induced autophagy.

Original languageEnglish
Pages (from-to)581-586
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume407
Issue number3
DOIs
StatePublished - Apr 15 2011

Fingerprint

Annexin A1
Starvation
Proteomics
Autophagy
Degradation
Proteins
HCT116 Cells
Proteome
Small Interfering RNA
Organelles
Salts
Cells
Cell Survival
Messenger RNA

Keywords

  • Annexin A1
  • Autophagy
  • Proteomics
  • Starvation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Proteomics analysis of starved cells revealed Annexin A1 as an important regulator of autophagic degradation. / Kang, Jeong Han; Li, Min; Chen, Xi; Yin, Xiao-Ming.

In: Biochemical and Biophysical Research Communications, Vol. 407, No. 3, 15.04.2011, p. 581-586.

Research output: Contribution to journalArticle

@article{01467f3cc9fa4b1ab8e2aec608678572,
title = "Proteomics analysis of starved cells revealed Annexin A1 as an important regulator of autophagic degradation",
abstract = "Macroautophagy is involved in the bulk degradation of long-lived cytosolic proteins and subcellular organelles, which is important for the survival of cells during starvation. To identify potential players of the autophagy process, we subjected HCT116 cells cultured in complete medium and in Earle's balanced salt solution to proteomics analysis. In approximately 1500 protein spots detected, we characterized 52 unique proteins, whose expression levels were significantly changed following starvation. Notably, we found that Annexin A1 was significantly upregulated following starvation at both mRNA and protein levels. Inhibition of Annexin A1 expression with specific siRNA did not alter starvation-induced autophagy as measured by the level of lipidated LC3, but significantly reversed autophagy degradation as measured by the level of p62/SQSTM 1. Thus Annexin A1 seemed to be positively upregulated during starvation to promote autophagic degradation. Overall, the data presented in this study established a expression profile of the proteome in starved cells, which allowed the identification of proteins with potential significance in starvation-induced autophagy.",
keywords = "Annexin A1, Autophagy, Proteomics, Starvation",
author = "Kang, {Jeong Han} and Min Li and Xi Chen and Xiao-Ming Yin",
year = "2011",
month = "4",
day = "15",
doi = "10.1016/j.bbrc.2011.03.067",
language = "English",
volume = "407",
pages = "581--586",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Proteomics analysis of starved cells revealed Annexin A1 as an important regulator of autophagic degradation

AU - Kang, Jeong Han

AU - Li, Min

AU - Chen, Xi

AU - Yin, Xiao-Ming

PY - 2011/4/15

Y1 - 2011/4/15

N2 - Macroautophagy is involved in the bulk degradation of long-lived cytosolic proteins and subcellular organelles, which is important for the survival of cells during starvation. To identify potential players of the autophagy process, we subjected HCT116 cells cultured in complete medium and in Earle's balanced salt solution to proteomics analysis. In approximately 1500 protein spots detected, we characterized 52 unique proteins, whose expression levels were significantly changed following starvation. Notably, we found that Annexin A1 was significantly upregulated following starvation at both mRNA and protein levels. Inhibition of Annexin A1 expression with specific siRNA did not alter starvation-induced autophagy as measured by the level of lipidated LC3, but significantly reversed autophagy degradation as measured by the level of p62/SQSTM 1. Thus Annexin A1 seemed to be positively upregulated during starvation to promote autophagic degradation. Overall, the data presented in this study established a expression profile of the proteome in starved cells, which allowed the identification of proteins with potential significance in starvation-induced autophagy.

AB - Macroautophagy is involved in the bulk degradation of long-lived cytosolic proteins and subcellular organelles, which is important for the survival of cells during starvation. To identify potential players of the autophagy process, we subjected HCT116 cells cultured in complete medium and in Earle's balanced salt solution to proteomics analysis. In approximately 1500 protein spots detected, we characterized 52 unique proteins, whose expression levels were significantly changed following starvation. Notably, we found that Annexin A1 was significantly upregulated following starvation at both mRNA and protein levels. Inhibition of Annexin A1 expression with specific siRNA did not alter starvation-induced autophagy as measured by the level of lipidated LC3, but significantly reversed autophagy degradation as measured by the level of p62/SQSTM 1. Thus Annexin A1 seemed to be positively upregulated during starvation to promote autophagic degradation. Overall, the data presented in this study established a expression profile of the proteome in starved cells, which allowed the identification of proteins with potential significance in starvation-induced autophagy.

KW - Annexin A1

KW - Autophagy

KW - Proteomics

KW - Starvation

UR - http://www.scopus.com/inward/record.url?scp=79954597240&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79954597240&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2011.03.067

DO - 10.1016/j.bbrc.2011.03.067

M3 - Article

VL - 407

SP - 581

EP - 586

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -