Proteomics: Technologies and applications

Mark P. Molloy, Frank A. Witzmann

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Enabling technologies for proteomics (studies examining the protein complement of the genome) have been in development for over 20 years. However, more recently, the field has become formalised by combining the techniques for large-scale protein separation (two-dimensional electrophoresis) with very precise, high fidelity approaches to the analysis and characterisation of the separated proteins (mass spectrometry). These approaches bring to reality the powerful scope of proteomics, enabling the interpretation of cell physiology through profiling the molecular biodescriptors of gene expression, the proteins. Applications of proteomics are wide ranging and can be found within many disciplines, although one of the more developed fields that is examined here covers the arena of toxicological and pharmacological profiling. In this paper, the current technologies that make proteomics possible will be looked at Some areas where proteomics has delivered valuable biological insight will also be considered, and there will be a look towards future areas of development.

Original languageEnglish (US)
Pages (from-to)23-39
Number of pages17
JournalBriefings in Functional Genomics and Proteomics
Volume1
Issue number1
DOIs
StatePublished - Feb 1 2002

Keywords

  • Moss spectometry
  • Proteomics
  • Technologies
  • Toxicology
  • Two-dimensional electrphoresis

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biochemistry

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