Providing CLARITY: 3D imaging of biomarkers in tumor samples

Yi Chen, Qi Shen, Yesim Polar, Sunil Badve, Laurie J. Goodman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The CLARITY method was used to transform core needle biopsies from patients with breast cancer, into optically transparent tissues, followed by multiplexed immunostaining and 3D imaging of key biomarkers. This data was compared to conventional FFPE thin-section methods. Manual pathology scoring of the 3D images from CLARITY processed samples demonstrated concordant results when compared to a set of 2D images scored throughout a similar sized FFPE block from the same patient.

Original languageEnglish (US)
Title of host publicationMicroscopy Histopathology and Analytics, MICROSCOPY 2018
PublisherOSA - The Optical Society
VolumePart F89-MICROSCOPY 2018
ISBN (Electronic)9781557528209
DOIs
StatePublished - Jan 1 2018
EventMicroscopy Histopathology and Analytics, MICROSCOPY 2018 - Hollywood, United States
Duration: Apr 3 2018Apr 6 2018

Other

OtherMicroscopy Histopathology and Analytics, MICROSCOPY 2018
CountryUnited States
CityHollywood
Period4/3/184/6/18

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Mechanics of Materials

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  • Cite this

    Chen, Y., Shen, Q., Polar, Y., Badve, S., & Goodman, L. J. (2018). Providing CLARITY: 3D imaging of biomarkers in tumor samples. In Microscopy Histopathology and Analytics, MICROSCOPY 2018 (Vol. Part F89-MICROSCOPY 2018). OSA - The Optical Society. https://doi.org/10.1364/MICROSCOPY.2018.MF3A.1