Proximal tubule characteristics of cultured human renal cortex epithelium

Stephen A. Kempson, James A. McAteer, Hasan A. Al-Mahrouq, Thomas P. Dousa, George S. Dougherty, Andrew Evan

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Cultured human renal cortical epithelial cells (NHK-C) were examined for functional and morphologic characteristics of the proximal tubule. Cultures were established by using cells isolated by progressive enzymatic dissociation from the extreme outer cortex of the normal human kidney. Cells were subcultured and used at passages 3 through 8. Cell uptake of α-methyl-d-glucoside (AMG), inorganic phosphate (Pi) and l-alanine was found to be dependent on the presence of Na+ in the incubation medium, and uptake increased with incubation time up to 30 minutes. Na+-dependent AMG uptake was inhibited 67% by phlorizin (1 mmol/L), and Pl uptake was inhibited 89% by parathyroid hormone (PTH) (10-6 mol/L). Intracellular cyclic adenosine monophosphate was increased 28-fold after exposure to 10-6 mol/L PTH but was increased only 2-fold by the same concentration of vasopressin. The cells exhibited endocytotic activity and possessed maltase, leucine aminopeptidase, and γ-glutamyltranspeptidase, enzymes located exclusively in the brush border membranes of proximal tubule cells. NHK-C cultures were structurally heterogeneous, made up of a mixed-cell population with predominant epithelial-like morphology. Epithelial cells had cuboidal form, solitary cilia, and short, irregularly distributed apical microvilli. These cultures also formed multicellular hemicysts, but only through passage 3. NHK-C cultures showed a dramatic attenuation of prollferative activity at passages 8 through 10. These data show that subcultured cells derived from the outer cortex of the normal human kidney retain a number of functional characteristics typical of the proximal tubule.

Original languageEnglish
Pages (from-to)278-284
Number of pages7
JournalJournal of Laboratory and Clinical Medicine
Volume113
Issue number3
StatePublished - 1989

Fingerprint

Parathyroid Hormone
Epithelium
Phlorhizin
Leucyl Aminopeptidase
Kidney
alpha-Glucosidases
Glucosides
Brushes
Vasopressins
Alanine
Cyclic AMP
Phosphates
Cells
Membranes
Microvilli
Enzymes
Epithelial Cells
Cilia
Population

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Kempson, S. A., McAteer, J. A., Al-Mahrouq, H. A., Dousa, T. P., Dougherty, G. S., & Evan, A. (1989). Proximal tubule characteristics of cultured human renal cortex epithelium. Journal of Laboratory and Clinical Medicine, 113(3), 278-284.

Proximal tubule characteristics of cultured human renal cortex epithelium. / Kempson, Stephen A.; McAteer, James A.; Al-Mahrouq, Hasan A.; Dousa, Thomas P.; Dougherty, George S.; Evan, Andrew.

In: Journal of Laboratory and Clinical Medicine, Vol. 113, No. 3, 1989, p. 278-284.

Research output: Contribution to journalArticle

Kempson, SA, McAteer, JA, Al-Mahrouq, HA, Dousa, TP, Dougherty, GS & Evan, A 1989, 'Proximal tubule characteristics of cultured human renal cortex epithelium', Journal of Laboratory and Clinical Medicine, vol. 113, no. 3, pp. 278-284.
Kempson SA, McAteer JA, Al-Mahrouq HA, Dousa TP, Dougherty GS, Evan A. Proximal tubule characteristics of cultured human renal cortex epithelium. Journal of Laboratory and Clinical Medicine. 1989;113(3):278-284.
Kempson, Stephen A. ; McAteer, James A. ; Al-Mahrouq, Hasan A. ; Dousa, Thomas P. ; Dougherty, George S. ; Evan, Andrew. / Proximal tubule characteristics of cultured human renal cortex epithelium. In: Journal of Laboratory and Clinical Medicine. 1989 ; Vol. 113, No. 3. pp. 278-284.
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