PTH-responsive osteoblast nuclear matrix architectural transcription factor binds to the rat type I collagen promoter

Marta Alvarez, Pasutha Thunyakitpisal, Paul Morrison, Jude Onyia, Janet Hock, Joseph P. Bidwell

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In connective tissue, cell structure contributes to type I collagen expression. Differences in osteoblast microarchitecture may account for the two distinct cis elements regulating basal expression, in vivo and in vitro, of the rat type I collagen α1(I) polypeptide chain (COL1A1). The COL1A1 promoter conformation may be the penultimate culmination of osteoblast structure. Architectural transcription factors bind to the minor groove of AT-rich DNA and bend it, altering interactions between other transacting proteins. Similarly, nuclear matrix (NM) proteins bind to the minor groove of AT-rich matrix-attachment regions, regulating transcription by altering DNA structure. We propose that osteoblast NM architectural transcription factors link cell structure to promoter geometry and COL1A1 transcription. Our objective was to identify potential osteoblast NM architectural transcription factors near the in vitro and in vivo regulatory regions of the rat COL1A1 promoter. Nuclear protein-promoter interactions were analyzed by gel shift analysis and related techniques. NM extracts were derived from rat osteosarcoma cells and from rat bone. The NM protein, NMP4, and a soluble nuclear protein, NP, both bound to two homologous poly(dT) elements within the COL1A1 in vitro regulatory region and proximal to the in vivo regulatory element. These proteins bound within the minor groove and bent the DNA. Parathyroid hormone increased NP/NMP4 binding to both poly(dT) elements and decreased COL1A1 mRNA in the osteosarcoma cells. NP/NMP4-COL1A1 promoter interactions may represent a molecular pathway by which osteoblast structure is coupled to COL1A1 expression.

Original languageEnglish (US)
Pages (from-to)336-352
Number of pages17
JournalJournal of Cellular Biochemistry
Volume69
Issue number3
DOIs
StatePublished - Jun 1 1998

Fingerprint

Nuclear Matrix
Osteoblasts
Collagen Type I
Rats
Transcription Factors
Nuclear Matrix-Associated Proteins
Poly T
Nucleic Acid Regulatory Sequences
Osteosarcoma
Transcription
Nuclear Proteins
DNA
Matrix Attachment Regions
Electrophoretic Mobility Shift Assay
Parathyroid Hormone
Connective Tissue
Conformations
Bone
Proteins
Gels

Keywords

  • Architectural transcription factor
  • Nuclear matrix
  • Osteoblast
  • Parathyroid hormone
  • Type collagen

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

PTH-responsive osteoblast nuclear matrix architectural transcription factor binds to the rat type I collagen promoter. / Alvarez, Marta; Thunyakitpisal, Pasutha; Morrison, Paul; Onyia, Jude; Hock, Janet; Bidwell, Joseph P.

In: Journal of Cellular Biochemistry, Vol. 69, No. 3, 01.06.1998, p. 336-352.

Research output: Contribution to journalArticle

Alvarez, Marta ; Thunyakitpisal, Pasutha ; Morrison, Paul ; Onyia, Jude ; Hock, Janet ; Bidwell, Joseph P. / PTH-responsive osteoblast nuclear matrix architectural transcription factor binds to the rat type I collagen promoter. In: Journal of Cellular Biochemistry. 1998 ; Vol. 69, No. 3. pp. 336-352.
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