PU.1 exhibits partial functional redundancy with Spi-B, but not with Ets-1 or Elf-1

Lee Ann Garrett-Sinha, Richard Dahl, Sridhar Rao, Kevin P. Barton, M. Celeste Simon

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44 Scopus citations

Abstract

Previously it was shown that the Ets proteins, PU.1 and Spi-B, exhibit functional redundancy in B lymphocytes. To investigate the possibility that PU.1 or Spi-B or both share overlapping roles with Ets-1 or Elf-1, PU.1+/-Ets-1-/-, PU.1+/-Elf-1-/-, and Spi-B-/-ETS-1-/- animals were generated. No blood cell defects were observed in these animals except those previously reported for Ets-1-/- mice. Therefore, no genetic overlap was detected between PU.1 or Spi-B with Ets-1 or Elf-1. In contrast, the results confirmed functional redundancy for PU.1 and Spi-B in that PU.1+/-Spi-B-/- bone marrow progenitors yielded smaller colonies in methylcellulose cultures than did wild-type, PU.1+/- or Spi- B-/- progenitors. In addition, PU.1+/-Spi- B+/+, PU.1+/-Spi-B+/-, and PU.1+/- Spi-B-/- mice displayed extramedullary splenic hematopoiesis. In summary, PU.1 and Spi-B regulate common target genes required for proliferation of hematopoietic progenitors or their committed descendants, whereas Ets-1 or Elf-1 do not appear to regulate shared target genes with PU.1 or Spi-B.

Original languageEnglish (US)
Pages (from-to)2908-2912
Number of pages5
JournalBlood
Volume97
Issue number9
DOIs
StatePublished - May 1 2001

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ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Garrett-Sinha, L. A., Dahl, R., Rao, S., Barton, K. P., & Celeste Simon, M. (2001). PU.1 exhibits partial functional redundancy with Spi-B, but not with Ets-1 or Elf-1. Blood, 97(9), 2908-2912. https://doi.org/10.1182/blood.V97.9.2908