PU.1 exhibits partial functional redundancy with Spi-B, but not with Ets-1 or Elf-1

Lee Ann Garrett-Sinha, Richard Dahl, Sridhar Rao, Kevin P. Barton, M. Celeste Simon

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Previously it was shown that the Ets proteins, PU.1 and Spi-B, exhibit functional redundancy in B lymphocytes. To investigate the possibility that PU.1 or Spi-B or both share overlapping roles with Ets-1 or Elf-1, PU.1+/-Ets-1-/-, PU.1+/-Elf-1-/-, and Spi-B-/-ETS-1-/- animals were generated. No blood cell defects were observed in these animals except those previously reported for Ets-1-/- mice. Therefore, no genetic overlap was detected between PU.1 or Spi-B with Ets-1 or Elf-1. In contrast, the results confirmed functional redundancy for PU.1 and Spi-B in that PU.1+/-Spi-B-/- bone marrow progenitors yielded smaller colonies in methylcellulose cultures than did wild-type, PU.1+/- or Spi- B-/- progenitors. In addition, PU.1+/-Spi- B+/+, PU.1+/-Spi-B+/-, and PU.1+/- Spi-B-/- mice displayed extramedullary splenic hematopoiesis. In summary, PU.1 and Spi-B regulate common target genes required for proliferation of hematopoietic progenitors or their committed descendants, whereas Ets-1 or Elf-1 do not appear to regulate shared target genes with PU.1 or Spi-B.

Original languageEnglish (US)
Pages (from-to)2908-2912
Number of pages5
JournalBlood
Volume97
Issue number9
DOIs
StatePublished - May 1 2001
Externally publishedYes

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Redundancy
Animals
Genes
Extramedullary Hematopoiesis
Methylcellulose
Lymphocytes
Blood Cells
Bone
Blood
B-Lymphocytes
Bone Marrow
Cells
Defects
proto-oncogene protein Spi-1
lambda Spi-1

ASJC Scopus subject areas

  • Hematology

Cite this

Garrett-Sinha, L. A., Dahl, R., Rao, S., Barton, K. P., & Celeste Simon, M. (2001). PU.1 exhibits partial functional redundancy with Spi-B, but not with Ets-1 or Elf-1. Blood, 97(9), 2908-2912. https://doi.org/10.1182/blood.V97.9.2908

PU.1 exhibits partial functional redundancy with Spi-B, but not with Ets-1 or Elf-1. / Garrett-Sinha, Lee Ann; Dahl, Richard; Rao, Sridhar; Barton, Kevin P.; Celeste Simon, M.

In: Blood, Vol. 97, No. 9, 01.05.2001, p. 2908-2912.

Research output: Contribution to journalArticle

Garrett-Sinha, LA, Dahl, R, Rao, S, Barton, KP & Celeste Simon, M 2001, 'PU.1 exhibits partial functional redundancy with Spi-B, but not with Ets-1 or Elf-1', Blood, vol. 97, no. 9, pp. 2908-2912. https://doi.org/10.1182/blood.V97.9.2908
Garrett-Sinha, Lee Ann ; Dahl, Richard ; Rao, Sridhar ; Barton, Kevin P. ; Celeste Simon, M. / PU.1 exhibits partial functional redundancy with Spi-B, but not with Ets-1 or Elf-1. In: Blood. 2001 ; Vol. 97, No. 9. pp. 2908-2912.
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