PU.1 expression in T follicular helper cells limits CD40L-dependent germinal center B cell development

Olufolakemi Awe, Matthew M. Hufford, Hao Wu, Duy Pham, Hua Chen Chang, Rukhsana Jabeen, Alexander Dent, Mark Kaplan

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

PU.1 is an ETS family transcription factor that is important for the development of multiple hematopoietic cell lineages. Previous work demonstrated a critical role for PU.1 in promoting Th9 development and in limiting Th2 cytokine production. Whether PU.1 has functions in other Th lineages is not clear. In this study, we examined the effects of ectopic expression of PU.1 in CD4+ T cells and observed decreased expression of genes involved with the function of T follicular helper (Tfh) cells, including Il21 and Tnfsf5 (encoding CD40L). T cells from conditional mutant mice that lack expression of PU.1 in T cells (Sfpi1lck-/-) demonstrated increased production of CD40L and IL-21 in vitro. Following adjuvant-dependent or adjuvant-independent immunization, we observed that Sfpi1lck-/- mice had increased numbers of Tfh cells, increased germinal center B cells (GCB cells), and increased Ab production in vivo. This correlated with increased expression of IL-21 and CD40L in Tfh cells from Sfpi1lck-/- mice compared with control mice. Finally, although blockade of IL-21 did not affect GCB cells in Sfpi1lck-/- mice, anti-CD40L treatment of immunized Sfpi1lck-/- mice decreased GCB cell numbers and Ag-specific Ig concentrations. Together, these data indicate an inhibitory role for PU.1 in the function of Tfh cells, germinal centers, and Tfh-dependent humoral immunity.

Original languageEnglish (US)
Pages (from-to)3705-3715
Number of pages11
JournalJournal of Immunology
Volume195
Issue number8
DOIs
StatePublished - Oct 15 2015

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CD40 Ligand
Germinal Center
Helper-Inducer T-Lymphocytes
B-Lymphocytes
T-Lymphocytes
Cell Lineage
Humoral Immunity
Immunization
Transcription Factors
Cell Count
Cytokines
Gene Expression
interleukin-21

ASJC Scopus subject areas

  • Immunology

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PU.1 expression in T follicular helper cells limits CD40L-dependent germinal center B cell development. / Awe, Olufolakemi; Hufford, Matthew M.; Wu, Hao; Pham, Duy; Chang, Hua Chen; Jabeen, Rukhsana; Dent, Alexander; Kaplan, Mark.

In: Journal of Immunology, Vol. 195, No. 8, 15.10.2015, p. 3705-3715.

Research output: Contribution to journalArticle

Awe, Olufolakemi ; Hufford, Matthew M. ; Wu, Hao ; Pham, Duy ; Chang, Hua Chen ; Jabeen, Rukhsana ; Dent, Alexander ; Kaplan, Mark. / PU.1 expression in T follicular helper cells limits CD40L-dependent germinal center B cell development. In: Journal of Immunology. 2015 ; Vol. 195, No. 8. pp. 3705-3715.
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