Pulmonary ischemia induces lung remodeling and angiogenesis

Elizabeth M. Wagner, Irina Petrache, Brian Schofield, Wayne Mitzner

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Cellular remodeling during angiogenesis in the lung is poorly described. Furthermore, it is the systemic vasculature of the lung and surrounding the lung that is proangiogenic when the pulmonary circulation becomes impaired. In a mouse model of chronic pulmonary thromboembolism, after left pulmonary artery ligation (LPAL), the intercostal vasculature, in proximity to the ischemic lung, proliferates and invades the lung (12). In the present study, we performed a detailed investigation of the kinetics of remodeling using histological sections of the left lung of C57Bl/6J mice after LPAL (4 h to 20 days) or after sham surgery. New vessels were seen within the thickened visceral pleura 4 days after LPAL predominantly in the upper portion of the left lung. Connections between new vessels within the pleura and pulmonary capillaries were clearly discerned by 7 days after LPAL. The visceral pleura and the lung parenchyma showed intense tissue remodeling, as evidenced by markedly elevated levels of both proliferating cell nuclear antigen and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling positive cells. Rapidly dividing cells were predominantly macrophages and type II pneumocytes. The increased apoptotic activity was further quantified by caspase-3 activity, which showed a sixfold increase relative to naive lungs, by 24 h after LPAL. Because sham surgeries had little effect on measured parameters, we conclude that both thoracic wound healing and pulmonary ischemia are required for systemic neovascularization.

Original languageEnglish (US)
Pages (from-to)587-593
Number of pages7
JournalJournal of Applied Physiology
Volume100
Issue number2
DOIs
StatePublished - Feb 2006
Externally publishedYes

Fingerprint

Ischemia
Lung
Pulmonary Artery
Ligation
Pleura
Alveolar Epithelial Cells
Pulmonary Circulation
DNA Nucleotidylexotransferase
Proliferating Cell Nuclear Antigen
Pulmonary Embolism
Caspase 3
Wound Healing
Thorax
Macrophages

Keywords

  • Apoptosis
  • Caspase-3
  • Histology

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Pulmonary ischemia induces lung remodeling and angiogenesis. / Wagner, Elizabeth M.; Petrache, Irina; Schofield, Brian; Mitzner, Wayne.

In: Journal of Applied Physiology, Vol. 100, No. 2, 02.2006, p. 587-593.

Research output: Contribution to journalArticle

Wagner, Elizabeth M. ; Petrache, Irina ; Schofield, Brian ; Mitzner, Wayne. / Pulmonary ischemia induces lung remodeling and angiogenesis. In: Journal of Applied Physiology. 2006 ; Vol. 100, No. 2. pp. 587-593.
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