Pulmonary microcirculatory kinetics of neutrophils deficient in leukocyte adhesion-promoting glycoproteins

Mervin Yoder, L. L. Checkley, U. Giger, W. L. Hanson, K. R. Kirk, R. L. Capen, W. W. Wagner

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The mechanism that causes neutrophils to sequester in the pulmonary circulation is unknown. Because the CD11/CD18 glycoprotein family on the surface membrane of neutrophils participates in many adhesive interactions with the endothelium, we investigated the role of these proteins in the intravascular sequestration of pulmonary neutrophils. Neutrophils were isolated from normal dogs and from the only living dog known to have leukocyte adhesion deficiency disease, an inherited deficiency of the CD11/CD18 adhesion family. The neutrophils were labeled with fluorescein dye, injected into normal recipient dogs, and their passage through the pulmonary microcirculation was recorded by in vivo videofluorescence microscopy through a transparent thoracic window. Transit times for normal and deficient neutrophils were similar over a wide range of hemodynamic conditions. Activation by zymosan-activated plasma, which increases the surface membrane expression of CD11/CD18, prolonged the transit of normal neutrophils but did not alter the transit time of the deficient neutrophils. These results indicate that neutrophil CD11/CD18 adhesion-promoting glycoproteins are not involved in the normal pulmonary sequestration of neutrophils but have a significant role in the arrest of activated neutrophils in the pulmonary capillaries.

Original languageEnglish
Pages (from-to)207-213
Number of pages7
JournalJournal of Applied Physiology
Volume69
Issue number1
StatePublished - 1990

Fingerprint

Glycoproteins
Neutrophils
Leukocytes
Lung
Bronchopulmonary Sequestration
Dogs
Deficiency Diseases
Zymosan
Pulmonary Circulation
Membranes
Microcirculation
Fluorescein
Adhesives
Endothelium
Coloring Agents
Thorax
Hemodynamics

Keywords

  • CD11/CD18
  • Dogs
  • Fluorescein-labeled neutrophils
  • Neutrophil kinetics
  • Neutrophil margination
  • Neutrophil transit times
  • Pulmonary neutrophils

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Yoder, M., Checkley, L. L., Giger, U., Hanson, W. L., Kirk, K. R., Capen, R. L., & Wagner, W. W. (1990). Pulmonary microcirculatory kinetics of neutrophils deficient in leukocyte adhesion-promoting glycoproteins. Journal of Applied Physiology, 69(1), 207-213.

Pulmonary microcirculatory kinetics of neutrophils deficient in leukocyte adhesion-promoting glycoproteins. / Yoder, Mervin; Checkley, L. L.; Giger, U.; Hanson, W. L.; Kirk, K. R.; Capen, R. L.; Wagner, W. W.

In: Journal of Applied Physiology, Vol. 69, No. 1, 1990, p. 207-213.

Research output: Contribution to journalArticle

Yoder, M, Checkley, LL, Giger, U, Hanson, WL, Kirk, KR, Capen, RL & Wagner, WW 1990, 'Pulmonary microcirculatory kinetics of neutrophils deficient in leukocyte adhesion-promoting glycoproteins', Journal of Applied Physiology, vol. 69, no. 1, pp. 207-213.
Yoder, Mervin ; Checkley, L. L. ; Giger, U. ; Hanson, W. L. ; Kirk, K. R. ; Capen, R. L. ; Wagner, W. W. / Pulmonary microcirculatory kinetics of neutrophils deficient in leukocyte adhesion-promoting glycoproteins. In: Journal of Applied Physiology. 1990 ; Vol. 69, No. 1. pp. 207-213.
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