Purification and cardiovascular activity of [Met1, Met5]-bradykinin from the plasma of a sturgeon (Acipenseriformes)

Zhihong Li, Michael P. Smith, Douglas W. Duff, Bruce A. Barton, Kenneth R. Olson, J. Michael Conlon

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The sturgeons (Order Acipenseriformes) are extant representatives of a group of primitive Actinopterygian (ray-finned) fish that probably shared a common ancestor with present-day teleosts. Incubation of heat-denatured plasma from a sturgeon (a hybrid of the shovelnosed sturgeon Scaphirhynchus platorynchus and the pallid sturgeon Scaphirhynchus albus) with either trypsin or porcine pancreatic kallikrein generated bradykinin-like immunoreactivity. The primary structure of sturgeon bradykinin was established as Met-Pro-Pro-Gly-Met-Ser-Pro-Phe-Arg. This amino acid sequence contains two amino acid substitutions (Arg1 → Met and Phe5 → Met) compared with mammalian bradykinin. Bolus injections of synthetic sturgeon bradykinin in doses as low as 1 pmol/kg into the dorsal aorta of unanesthetized sturgeon resulted in an immediate and significant fall in arterial blood pressure with a maximum depressor response at 300 pmol/kg. Thus, the cardiovascular response of the sturgeon to bradykinin resembles more closely the response of mammals rather than the predominantly pressor response seen in teleost fish. Sturgeon bradykinin produced a strong and concentration-dependent (EC50 = 4.7 ± 0.7 x 10-10 M) relaxation of rings of vascular tissue from the sturgeon ventral aorta that had been pre- contracted with acetylcholine. The data indicate that sturgeon tissues are particularly responsive to native bradykinin and suggest that the kallikrein- kinin system may have evolved before the appearance of the neopterygians (gars, bowfin and teleosts).

Original languageEnglish (US)
Pages (from-to)635-641
Number of pages7
JournalPeptides
Volume19
Issue number4
DOIs
StatePublished - Apr 1 1998

Keywords

  • Arterial blood pressure
  • Bradykinin
  • Chondrostean

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

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