Purification and characterization of mouse alcohol dehydrogenase from two inbred strains that differ in total liver enzyme activity

Douglas Rex, William F. Bosron, Ting Kai Li

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Alcohol dehydrogenase activity in mouse liver homogenate-supernatants is 1.7 times greater in the C57BL/10 strain than in the BALB/c strain, regardless of whether activity is expressed in units per gram liver, total liver, or milligram DNA. The Km values for ethanol and NAD+, approximately 0.4 and 0.03 mm, respectively, of enzyme purified from both strains are similar. Moreover, the Ki for NADH, 1 μm, the pH optimum for ethanol oxidation, 10.5, and the Vmax for ethanol oxidation, 160 min-1, for ADH from the C57BL/10 and BALB/c strains are similar. Therefore, the difference in ADH activity in the two strains cannot be due to differences in the catalytic properties of the enzyme. The electrophoretic and isoelectric focusing patterns and two-dimensional tryptic peptide maps of the purified enzyme from both strains are identical. Thus the amino acid sequences of enzyme from C57BL/10 and BALB/c mice must also be identical or very similar. The difference in ADH activity in the two strains is most likely the result of genetic differences in the content of ADH protein in liver.

Original languageEnglish
Pages (from-to)115-124
Number of pages10
JournalBiochemical Genetics
Volume22
Issue number1-2
DOIs
StatePublished - Feb 1984

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Alcohol Dehydrogenase
alcohol dehydrogenase
Enzyme activity
Liver
enzyme activity
Purification
purification
alcohol
enzyme
ethanol
liver
Ethanol
mice
Enzymes
NAD
oxidation
Oxidation
enzymes
Isoelectric Focusing
peptide

Keywords

  • alcohol dehydrogenase
  • enzyme structure
  • inbred mouse strains
  • kinetics

ASJC Scopus subject areas

  • Genetics
  • Biochemistry

Cite this

Purification and characterization of mouse alcohol dehydrogenase from two inbred strains that differ in total liver enzyme activity. / Rex, Douglas; Bosron, William F.; Li, Ting Kai.

In: Biochemical Genetics, Vol. 22, No. 1-2, 02.1984, p. 115-124.

Research output: Contribution to journalArticle

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