Purification and characterization of the d-mannose receptor from J774 mouse macrophage cells

Janice S. Blum, Philip D. Stahl, Ruben Diaz, Maria L. Fiani

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Macrophages display on their cell surface a d-mannose-specific receptor which facilitates the scavenging of certain pathogens and deleterious macromolecules from the extracellular fluid as part of the host defense mechanism. The mouse d-mannose receptor was purified from J774 E macrophages and an antiserum was generated against the receptor protein. In mouse macrophages, the newly synthesized receptor has an Mr of 157 000 Da and rapidly matures to a protein with an Mr of 172 000 Da. Both forms of the receptor protein are tightly associated with cell membranes. The receptor is found in a number of mouse macrophage cell types but is not present in mouse fibroblasts. An assay was developed to characterize d-mannose receptor-ligand binding based on immunoprecipitation of the detergent-solubilized receptor protein. The dissociation constant, determined for receptor and the neoglycoprotein d-mannose-BSA, was 1.67nm. Receptor-ligand binding was calcium and pH dependent. Monosaccharides, such as d-mannose and l-fucose, partially inhibited receptor binding to the ligand d-mannose-BSA.

Original languageEnglish (US)
Pages (from-to)145-153
Number of pages9
JournalCarbohydrate Research
Volume213
Issue numberC
DOIs
StatePublished - Jun 25 1991

Fingerprint

Macrophages
Purification
Mannose
Ligands
Proteins
Fucose
Monosaccharides
Extracellular Fluid
Scavenging
Pathogens
Fibroblasts
Cell membranes
Macromolecules
Immunoprecipitation
Detergents
Immune Sera
Assays
Glycoproteins
Cell Membrane
Calcium

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Purification and characterization of the d-mannose receptor from J774 mouse macrophage cells. / Blum, Janice S.; Stahl, Philip D.; Diaz, Ruben; Fiani, Maria L.

In: Carbohydrate Research, Vol. 213, No. C, 25.06.1991, p. 145-153.

Research output: Contribution to journalArticle

Blum, Janice S. ; Stahl, Philip D. ; Diaz, Ruben ; Fiani, Maria L. / Purification and characterization of the d-mannose receptor from J774 mouse macrophage cells. In: Carbohydrate Research. 1991 ; Vol. 213, No. C. pp. 145-153.
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