Purification and partial characterization of branched-chain α-ketoacid dehydrogenase kinase from rat liver and rat heart

Yoshiharu Shimomura, Noriko Nanaumi, Masashige Suzuki, Kirill M. Popov, Robert Harris

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Branched-chain α-ketoacid dehydrogenase kinase was purified to homogeneity from rat liver and rat heart. The initial step was the purification of rat liver and heart branched-chain α-ketoacid dehydrogenase complex with high kinase activity by a modification of a method described previously. Preservation of high kinase activity during purification of the complex required the presence of fresh dithiothreitol throughout the procedure. The kinase was released from the complex by oxidation of dithiothreitol with potassium ferricyanide and purified by high-speed centrifugation, immunoadsorption chromatography, and DEAE-Sephacel chromatography. Both kinase preparations gave only one polypeptide band with a molecular weight of 44,000 on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Phosphorylation and inactivation of the branched-chain α-ketoacid dehydrogenase complex by the purified kinase was inhibited by α-chloroisocaproate and dichloroacetate, established inhibitors of the phosphorylation of the branched-chain α-ketoacid dehydrogenase complex. The kinase did not exhibit autophosphorylation and does not correspond to the same protein as pyruvate dehydrogenase kinase. The kinase phosphorylated histone (type II-S), but this reaction was slow relative to the phosphorylation of the branched-chain α-ketoacid dehydrogenase complex and was not inhibited by α-chloroisocaproate.

Original languageEnglish
Pages (from-to)293-299
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume283
Issue number2
DOIs
StatePublished - 1990

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3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
Liver
Purification
Rats
Phosphotransferases
Phosphorylation
Dithiothreitol
Chromatography
Protamine Kinase
Centrifugation
Sodium Dodecyl Sulfate
Electrophoresis
Polyacrylamide Gel Electrophoresis
Histones
Molecular Weight
Molecular weight
Peptides
Oxidation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Purification and partial characterization of branched-chain α-ketoacid dehydrogenase kinase from rat liver and rat heart. / Shimomura, Yoshiharu; Nanaumi, Noriko; Suzuki, Masashige; Popov, Kirill M.; Harris, Robert.

In: Archives of Biochemistry and Biophysics, Vol. 283, No. 2, 1990, p. 293-299.

Research output: Contribution to journalArticle

Shimomura, Yoshiharu ; Nanaumi, Noriko ; Suzuki, Masashige ; Popov, Kirill M. ; Harris, Robert. / Purification and partial characterization of branched-chain α-ketoacid dehydrogenase kinase from rat liver and rat heart. In: Archives of Biochemistry and Biophysics. 1990 ; Vol. 283, No. 2. pp. 293-299.
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