Purification of a vasoactive peptide related to lysyl-bradykinin from trout plasma

J. Michael Conlon, Kenneth R. Olson

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Incubation of plasma from the steelhead trout, Oncorhynchus mykiss with porcine pancreatic glandular kallikrein generated bradykinin-like immunoreactivity. The primary structure of the immunoreactive peptide was established as: Lys-Arg-Pro-Pro-Gly-Trp-Ser-Pro-Leu-Arg. This sequence shows two amino acid substitutions (Phe6→Trp and Phe9→Leu) compared with mammalian lysyl-bradykinin (kallidin). Bolus intra-arterial injection of the purified peptide produced a strong and sustained vasopressor response in the unanaesthetized trout. The data demonstrate that the kallikrein-kinin system predates the appearance of tetrapods and suggest a role for this system in cardiovascular regulation in fish.

Original languageEnglish (US)
Pages (from-to)75-78
Number of pages4
JournalFEBS Letters
Volume334
Issue number1
DOIs
StatePublished - Nov 8 1993

Keywords

  • Blood pressure
  • Bradykinin
  • Kallidin
  • Kallikrein
  • Kininogen
  • Trout

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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