Purification of phospholamban from bovine cardiac muscle with organic solvents

P. Boyot, B. Luu, L. R. Jones, E. Trifilieff

Research output: Contribution to journalArticle

11 Scopus citations


Phospholamban (PLB), an integral membrane protein of cardiac sarcoplasmic reticulum, was extracted from bovine cardiac muscle with an acidic chloroform/methanol mixture. A combination of gel permeation and ion-exchange chromatographies in organic solvents allowed the purification of PLB. The intensive use of organic solvents throughout the isolation yielded a highly purified and intact protein that can be phosphorylated by cAMP protein kinase. The ease of purification and the high yield obtained (2.5 mg/100 g of fresh tissue) show that organic solvents can be very useful in the extraction and purification of hydrophobic membrane proteins.

Original languageEnglish (US)
Pages (from-to)639-645
Number of pages7
JournalArchives of Biochemistry and Biophysics
Issue number2
StatePublished - Mar 1989

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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