Purification of plasma vitamin D metabolites for radioimmunoassay

G. A. Taylor, M. Peacock, B. Pelc, W. Brown, A. Holmes

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

The ability of four solvent systems to extract tritiated 25-hydroxy vitamin D3, 24,25-dihydroxy vitamin D3, 25,26-dihydroxy vitamin D3 and 1α,25-dihydroxy vitamin D3 from plasma was compared. Diethyl ether gave the highest yield of metabolites and lowest yield of "lipid" materials, the dihydroxylated metabolites were more readily extracted than 25-hydroxy vitamin D3. Following extraction with ether the vitamin D metabolites could be separated, without prior purification, on a 6.2 mm × 250 mm Zorbax-Sil high pressure liquid chromatography (HPLC) column, a simplification of previous methods. Plasma 1α,25-dihydroxy vitamin D levels measured after purification by this method were not significantly different from those obtained by an established, more complex method.

Original languageEnglish (US)
Pages (from-to)239-246
Number of pages8
JournalClinica Chimica Acta
Volume108
Issue number2
DOIs
StatePublished - Dec 8 1980

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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    Taylor, G. A., Peacock, M., Pelc, B., Brown, W., & Holmes, A. (1980). Purification of plasma vitamin D metabolites for radioimmunoassay. Clinica Chimica Acta, 108(2), 239-246. https://doi.org/10.1016/0009-8981(80)90010-8