Purification, primary structure, and immunological characterization of the 26-kDa calsequestrin binding protein (junctin) from cardiac junctional sarcoplasmic reticulum

Larry Jones, Lin Zhang, Kristi Sanborn, Annelise O. Jorgensen, Jeff Kelley

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

Previously we identified a protein of apparent M(r) = 26,000 as the major calsequestrin binding protein in junctional sarcoplasmic reticulum vesicles isolated from cardiac and skeletal muscle (Mitchell, R. D., Simmerman, H. K. B, and Jones, L. R. (1988) J. Biol. Chem. 263, 1376-1381). Here we describe the purification and primary structure of the 26-kDa calsequestrin binding protein. The protein was purified 164-fold from cardiac microsomes and shown by immunoblotting to be highly enriched in junctional membrane subfractions. It ran as a closely spaced doublet on SDS-polyacrylamide gel electrophoresis and bound 125I-calsequestrin intensely. Cloning of the cDNA predicted a protein of 210 amino acids containing a single transmembrane domain. The protein has a short N-terminal region located in the cytoplasm, and the bulk of the molecule, which is highly charged and basic, projects into the sarcoplasmic reticulum lumen. Significant homologies were found with triadin and aspartyl β-hydroxylase, suggesting that all three proteins are members of a family of single membrane-spanning endoplasmic reticulum proteins. Immunocytochemical labeling localized the 26-kDa protein to junctional sarcoplasmic reticulum in cardiac and skeletal muscle. The same gene product was expressed in these two tissues. The calsequestrin binding activity of the 26-kDa protein combined with its codistribution with calsequestrin and ryanodine receptors strongly suggests that the protein plays an important role in the organization and/or function of the Ca2+ release complex. Because the 26-kDa calsequestrin binding protein is an integral component of the junctional sarcoplasmic reticulum membrane in cardiac and skeletal muscle, we have named it Junctin.

Original languageEnglish
Pages (from-to)30787-30796
Number of pages10
JournalJournal of Biological Chemistry
Volume270
Issue number51
DOIs
StatePublished - Dec 22 1995

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Calsequestrin
Sarcoplasmic Reticulum
Purification
Carrier Proteins
Proteins
Muscle
Myocardium
Skeletal Muscle
Membranes
Ryanodine Receptor Calcium Release Channel
Cloning
Microsomes
Mixed Function Oxygenases
Electrophoresis
Immunoblotting
Endoplasmic Reticulum
Labeling
Organism Cloning
Polyacrylamide Gel Electrophoresis
Cytoplasm

ASJC Scopus subject areas

  • Biochemistry

Cite this

Purification, primary structure, and immunological characterization of the 26-kDa calsequestrin binding protein (junctin) from cardiac junctional sarcoplasmic reticulum. / Jones, Larry; Zhang, Lin; Sanborn, Kristi; Jorgensen, Annelise O.; Kelley, Jeff.

In: Journal of Biological Chemistry, Vol. 270, No. 51, 22.12.1995, p. 30787-30796.

Research output: Contribution to journalArticle

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