Purified human transferrin and 'transferrin' released from sorted T8+ lymphocytes suppress release of granulocyte-macrophage colony-stimulating factors from sorted T4+ lymphocytes stimulated by phytohemagglutinin

L. Lu, D. C. Bicknell, W. Piacibello, H. E. Broxmeyer

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Nonadherent, low-density E-rosette-positive human peripheral blood cells were separated into T4+ and T8+ lymphocytes by immunofluorescence-activated cell sorting (FACS) with monoclonal antibodies OKT4 and OKT8. Both T4+ and T8+ lymphocytes released granulocyte-macrophage colony-stimulating factors (GM-CSF) in response to phytohemagglutinin (PHA). Purified iron-saturated human transferrin (TF) suppressed release of GM-CSF only from the T4+ subset of lymphocytes. A TF-type inhibitory activity was released from the T8+ subset of lymphocytes alone, and this inhibitory activity, as well as that in purified TF, was inactivated by preincubation with antihuman TF monoclonal antibody (HT/1). These studies suggest that, at least in vitro, subsets, of T-lymphocytes and TF may be involved in the regulation of myelopoiesis.

Original languageEnglish (US)
Pages (from-to)955-962
Number of pages8
JournalExperimental Hematology
Volume14
Issue number10
StatePublished - Dec 1 1986

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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