Purkinje cell age-distribution in fissures and in foliar crowns

A comparative study in the weaver cerebellum

Joaquín Martí, M. C. Santa-Cruz, Shirley A. Bayer, Bernardino Ghetti, José P. Hervás

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Generation and settling of Purkinje cells (PCs) are investigated in the weaver mouse cerebellum in order to determine possible relationships with the fissuration pattern. Tritiated thymidine was supplied to pregnant females at the time that these neurons were being produced. Autoradiography was then applied on brain sections obtained from control and weaver offspring at postnatal (P) day 90. This makes it possible to assess the differential survival of neurons born at distinct embryonic times on the basis of the proportion of labeled cells located at the two foliar compartments: fissures and foliar crowns. Our data show that throughout the surface contour of the vermal lobes, generative programs of PCs were close between wild type and homozygous weaver. Similar data were found in the lobules of the lateral hemisphere. On the other hand, the loss of PCs in weaver cerebella can be related to foliar concavities or convexities depending on the vermal lobe or the hemispheric lobule studied. Lastly, we have obtained evidence that late-generated PCs of both normal and mutant mice were preferentially located in fissures. These quantitative relationships lead us to propose a model in which the final distribution of PCs through the vermal contour would be coupled to two factors: the cortical fissuration patterning and a "time-sequential effect" of weaver mutation.

Original languageEnglish
Pages (from-to)347-357
Number of pages11
JournalBrain Structure and Function
Volume212
Issue number3-4
DOIs
StatePublished - Dec 2007

Fingerprint

Purkinje Cells
Age Distribution
Crowns
Cerebellum
Neurologic Mutant Mice
Neurons
Autoradiography
Thymidine
Mutation
Brain

Keywords

  • Autoradiography
  • Cerebellar cortex
  • Fissuration
  • Neurogenetic gradients
  • Purkinje cells
  • Weaver mice

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Neuroscience(all)

Cite this

Purkinje cell age-distribution in fissures and in foliar crowns : A comparative study in the weaver cerebellum. / Martí, Joaquín; Santa-Cruz, M. C.; Bayer, Shirley A.; Ghetti, Bernardino; Hervás, José P.

In: Brain Structure and Function, Vol. 212, No. 3-4, 12.2007, p. 347-357.

Research output: Contribution to journalArticle

Martí, Joaquín ; Santa-Cruz, M. C. ; Bayer, Shirley A. ; Ghetti, Bernardino ; Hervás, José P. / Purkinje cell age-distribution in fissures and in foliar crowns : A comparative study in the weaver cerebellum. In: Brain Structure and Function. 2007 ; Vol. 212, No. 3-4. pp. 347-357.
@article{34cc0169c6ea476d816612ec3683f024,
title = "Purkinje cell age-distribution in fissures and in foliar crowns: A comparative study in the weaver cerebellum",
abstract = "Generation and settling of Purkinje cells (PCs) are investigated in the weaver mouse cerebellum in order to determine possible relationships with the fissuration pattern. Tritiated thymidine was supplied to pregnant females at the time that these neurons were being produced. Autoradiography was then applied on brain sections obtained from control and weaver offspring at postnatal (P) day 90. This makes it possible to assess the differential survival of neurons born at distinct embryonic times on the basis of the proportion of labeled cells located at the two foliar compartments: fissures and foliar crowns. Our data show that throughout the surface contour of the vermal lobes, generative programs of PCs were close between wild type and homozygous weaver. Similar data were found in the lobules of the lateral hemisphere. On the other hand, the loss of PCs in weaver cerebella can be related to foliar concavities or convexities depending on the vermal lobe or the hemispheric lobule studied. Lastly, we have obtained evidence that late-generated PCs of both normal and mutant mice were preferentially located in fissures. These quantitative relationships lead us to propose a model in which the final distribution of PCs through the vermal contour would be coupled to two factors: the cortical fissuration patterning and a {"}time-sequential effect{"} of weaver mutation.",
keywords = "Autoradiography, Cerebellar cortex, Fissuration, Neurogenetic gradients, Purkinje cells, Weaver mice",
author = "Joaqu{\'i}n Mart{\'i} and Santa-Cruz, {M. C.} and Bayer, {Shirley A.} and Bernardino Ghetti and Herv{\'a}s, {Jos{\'e} P.}",
year = "2007",
month = "12",
doi = "10.1007/s00429-007-0159-8",
language = "English",
volume = "212",
pages = "347--357",
journal = "Anatomy and Embryology",
issn = "0177-5154",
publisher = "Springer Verlag",
number = "3-4",

}

TY - JOUR

T1 - Purkinje cell age-distribution in fissures and in foliar crowns

T2 - A comparative study in the weaver cerebellum

AU - Martí, Joaquín

AU - Santa-Cruz, M. C.

AU - Bayer, Shirley A.

AU - Ghetti, Bernardino

AU - Hervás, José P.

PY - 2007/12

Y1 - 2007/12

N2 - Generation and settling of Purkinje cells (PCs) are investigated in the weaver mouse cerebellum in order to determine possible relationships with the fissuration pattern. Tritiated thymidine was supplied to pregnant females at the time that these neurons were being produced. Autoradiography was then applied on brain sections obtained from control and weaver offspring at postnatal (P) day 90. This makes it possible to assess the differential survival of neurons born at distinct embryonic times on the basis of the proportion of labeled cells located at the two foliar compartments: fissures and foliar crowns. Our data show that throughout the surface contour of the vermal lobes, generative programs of PCs were close between wild type and homozygous weaver. Similar data were found in the lobules of the lateral hemisphere. On the other hand, the loss of PCs in weaver cerebella can be related to foliar concavities or convexities depending on the vermal lobe or the hemispheric lobule studied. Lastly, we have obtained evidence that late-generated PCs of both normal and mutant mice were preferentially located in fissures. These quantitative relationships lead us to propose a model in which the final distribution of PCs through the vermal contour would be coupled to two factors: the cortical fissuration patterning and a "time-sequential effect" of weaver mutation.

AB - Generation and settling of Purkinje cells (PCs) are investigated in the weaver mouse cerebellum in order to determine possible relationships with the fissuration pattern. Tritiated thymidine was supplied to pregnant females at the time that these neurons were being produced. Autoradiography was then applied on brain sections obtained from control and weaver offspring at postnatal (P) day 90. This makes it possible to assess the differential survival of neurons born at distinct embryonic times on the basis of the proportion of labeled cells located at the two foliar compartments: fissures and foliar crowns. Our data show that throughout the surface contour of the vermal lobes, generative programs of PCs were close between wild type and homozygous weaver. Similar data were found in the lobules of the lateral hemisphere. On the other hand, the loss of PCs in weaver cerebella can be related to foliar concavities or convexities depending on the vermal lobe or the hemispheric lobule studied. Lastly, we have obtained evidence that late-generated PCs of both normal and mutant mice were preferentially located in fissures. These quantitative relationships lead us to propose a model in which the final distribution of PCs through the vermal contour would be coupled to two factors: the cortical fissuration patterning and a "time-sequential effect" of weaver mutation.

KW - Autoradiography

KW - Cerebellar cortex

KW - Fissuration

KW - Neurogenetic gradients

KW - Purkinje cells

KW - Weaver mice

UR - http://www.scopus.com/inward/record.url?scp=36448988277&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36448988277&partnerID=8YFLogxK

U2 - 10.1007/s00429-007-0159-8

DO - 10.1007/s00429-007-0159-8

M3 - Article

VL - 212

SP - 347

EP - 357

JO - Anatomy and Embryology

JF - Anatomy and Embryology

SN - 0177-5154

IS - 3-4

ER -