Pyruvate dehydrogenase kinase-4 contributes to the recirculation of gluconeogenic precursors during postexercise glycogen recovery

Eric A F Herbst, Rebecca E K Macpherson, Paul J. Leblanc, Brian D. Roy, Nam Ho Jeoung, Robert Harris, Sandra J. Peters

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

During recovery from glycogen-depleting exercise, there is a shift from carbohydrate oxidation to glycogen resynthesis. The activity of the pyruvate dehydrogenase (PDH) complex may decrease to reduce oxidation of carbohydrates in favor of increasing gluconeogenic recycling of carbohydrate-derived substrates for this process. The precise mechanism behind this has yet to be elucidated; however, research examining mRNA content has suggested that the less-abundant pyruvate dehydrogenase kinase-4 (PDK4) may reduce PDH activation during exercise recovery. To investigate this, skeletal muscle and liver of wild-type (WT) and PDK4-knockout (PDK4-KO) mice were analyzed at rest (Rest), after exercise to exhaustion (Exh), and after 2 h of recovery with ad libitum feeding (Rec). Although there were no differences in exercise tolerance between genotypes, caloric consumption was doubled by PDK4-KO mice during Rec. Because of this, PDK4-KO mice at Rec supercompensated muscle glycogen to 120% of resting stores. Therefore, an extra group of PDK4-KO mice were pair-fed (PF) with WT mice during Rec for comparison. PF mice fully replenished muscle glycogen but recovered only 50% of liver glycogen stores. Concentrations of muscle lactate and alanine were also lower in PF than in WT mice, indicating that this decrease may lead to a potential reduction of recycled gluconeogenic substrates, due to oxidation of their carbohydrate precursors in skeletal muscle, leading to observed reductions in hepatic glucose and glycogen concentrations. Because of the impairments seen in PF PDK4-KO mice, these results suggest a role for PDK4 in regulating the PDH complex in muscle and promoting gluconeogenic precursor recirculation during recovery from exhaustive exercise.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume306
Issue number2
DOIs
StatePublished - Jan 15 2014

Fingerprint

Glycogen
Carbohydrates
Pyruvate Dehydrogenase Complex
Muscles
Liver Glycogen
Skeletal Muscle
Exercise Tolerance
Recycling
pyruvate dehydrogenase kinase 4
Pyruvic Acid
Knockout Mice
Alanine
Lactic Acid
Oxidoreductases
Genotype
Glucose
Messenger RNA
Liver
Research

Keywords

  • Exercise
  • Glycogen
  • Oxidative metabolism

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Pyruvate dehydrogenase kinase-4 contributes to the recirculation of gluconeogenic precursors during postexercise glycogen recovery. / Herbst, Eric A F; Macpherson, Rebecca E K; Leblanc, Paul J.; Roy, Brian D.; Jeoung, Nam Ho; Harris, Robert; Peters, Sandra J.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 306, No. 2, 15.01.2014.

Research output: Contribution to journalArticle

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