Quality testing of autoclaved rodent drinking water during short-term and long-term storage

Jessica L. Peveler, Robin Crisler, Deb Hickman

Research output: Contribution to journalArticlepeer-review


All animals need clean water to drink. At the authors' animal facility, drinking water for immunocompromised rodents is filtered by reverse osmosis, acidified during bottling and sterilized in an autoclave. Autoclaved water bottles can be stored in unopened autoclave bags for 7 d or in opened bags for 2 d; if not used during that time, they are emptied, cleaned, refilled and sterilized again. The authors wished to determine whether the storage period of 2-7 d was adequate and necessary to ensure the quality of drinking water. They tested water bottles for pH levels and for the presence of adenosine triphosphate as a measure of organic contamination during short-term and long-term storage. The pH of autoclaved drinking water generally remained stable during storage. Furthermore, no instances of organic contamination were detected in autoclaved water bottles stored for up to 22 d in unopened bags and only one instance was detected in bottles stored for up to 119 d in opened bags in a room with individually ventilated cages. On the basis of these findings, the acceptable storage period for autoclaved water bottles in opened bags at the authors' facility was extended to 21 d.

Original languageEnglish (US)
Pages (from-to)211-215
Number of pages5
JournalLab Animal
Issue number6
StatePublished - May 19 2015

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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