Quality testing of autoclaved rodent drinking water during short-term and long-term storage

Research output: Contribution to journalArticle

Abstract

All animals need clean water to drink. At the authors' animal facility, drinking water for immunocompromised rodents is filtered by reverse osmosis, acidified during bottling and sterilized in an autoclave. Autoclaved water bottles can be stored in unopened autoclave bags for 7 d or in opened bags for 2 d; if not used during that time, they are emptied, cleaned, refilled and sterilized again. The authors wished to determine whether the storage period of 2-7 d was adequate and necessary to ensure the quality of drinking water. They tested water bottles for pH levels and for the presence of adenosine triphosphate as a measure of organic contamination during short-term and long-term storage. The pH of autoclaved drinking water generally remained stable during storage. Furthermore, no instances of organic contamination were detected in autoclaved water bottles stored for up to 22 d in unopened bags and only one instance was detected in bottles stored for up to 119 d in opened bags in a room with individually ventilated cages. On the basis of these findings, the acceptable storage period for autoclaved water bottles in opened bags at the authors' facility was extended to 21 d.

Original languageEnglish (US)
Pages (from-to)211-215
Number of pages5
JournalLab Animal
Volume44
Issue number6
DOIs
StatePublished - May 19 2015

Fingerprint

bottles
Drinking Water
drinking water
bags
Rodentia
rodents
Water
autoclaves
water
testing
storage time
bottling
Osmosis
reverse osmosis
adenosine triphosphate
animals
cages
Adenosine Triphosphate

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Quality testing of autoclaved rodent drinking water during short-term and long-term storage. / Peveler, Jessica L.; Crisler-Roberts, Robin; Hickman, Debra.

In: Lab Animal, Vol. 44, No. 6, 19.05.2015, p. 211-215.

Research output: Contribution to journalArticle

@article{1434bc7dc3464dbaa64754cb38a296fa,
title = "Quality testing of autoclaved rodent drinking water during short-term and long-term storage",
abstract = "All animals need clean water to drink. At the authors' animal facility, drinking water for immunocompromised rodents is filtered by reverse osmosis, acidified during bottling and sterilized in an autoclave. Autoclaved water bottles can be stored in unopened autoclave bags for 7 d or in opened bags for 2 d; if not used during that time, they are emptied, cleaned, refilled and sterilized again. The authors wished to determine whether the storage period of 2-7 d was adequate and necessary to ensure the quality of drinking water. They tested water bottles for pH levels and for the presence of adenosine triphosphate as a measure of organic contamination during short-term and long-term storage. The pH of autoclaved drinking water generally remained stable during storage. Furthermore, no instances of organic contamination were detected in autoclaved water bottles stored for up to 22 d in unopened bags and only one instance was detected in bottles stored for up to 119 d in opened bags in a room with individually ventilated cages. On the basis of these findings, the acceptable storage period for autoclaved water bottles in opened bags at the authors' facility was extended to 21 d.",
author = "Peveler, {Jessica L.} and Robin Crisler-Roberts and Debra Hickman",
year = "2015",
month = "5",
day = "19",
doi = "10.1038/laban.734",
language = "English (US)",
volume = "44",
pages = "211--215",
journal = "Lab Animal",
issn = "0093-7355",
publisher = "Nature Publishing Group",
number = "6",

}

TY - JOUR

T1 - Quality testing of autoclaved rodent drinking water during short-term and long-term storage

AU - Peveler, Jessica L.

AU - Crisler-Roberts, Robin

AU - Hickman, Debra

PY - 2015/5/19

Y1 - 2015/5/19

N2 - All animals need clean water to drink. At the authors' animal facility, drinking water for immunocompromised rodents is filtered by reverse osmosis, acidified during bottling and sterilized in an autoclave. Autoclaved water bottles can be stored in unopened autoclave bags for 7 d or in opened bags for 2 d; if not used during that time, they are emptied, cleaned, refilled and sterilized again. The authors wished to determine whether the storage period of 2-7 d was adequate and necessary to ensure the quality of drinking water. They tested water bottles for pH levels and for the presence of adenosine triphosphate as a measure of organic contamination during short-term and long-term storage. The pH of autoclaved drinking water generally remained stable during storage. Furthermore, no instances of organic contamination were detected in autoclaved water bottles stored for up to 22 d in unopened bags and only one instance was detected in bottles stored for up to 119 d in opened bags in a room with individually ventilated cages. On the basis of these findings, the acceptable storage period for autoclaved water bottles in opened bags at the authors' facility was extended to 21 d.

AB - All animals need clean water to drink. At the authors' animal facility, drinking water for immunocompromised rodents is filtered by reverse osmosis, acidified during bottling and sterilized in an autoclave. Autoclaved water bottles can be stored in unopened autoclave bags for 7 d or in opened bags for 2 d; if not used during that time, they are emptied, cleaned, refilled and sterilized again. The authors wished to determine whether the storage period of 2-7 d was adequate and necessary to ensure the quality of drinking water. They tested water bottles for pH levels and for the presence of adenosine triphosphate as a measure of organic contamination during short-term and long-term storage. The pH of autoclaved drinking water generally remained stable during storage. Furthermore, no instances of organic contamination were detected in autoclaved water bottles stored for up to 22 d in unopened bags and only one instance was detected in bottles stored for up to 119 d in opened bags in a room with individually ventilated cages. On the basis of these findings, the acceptable storage period for autoclaved water bottles in opened bags at the authors' facility was extended to 21 d.

UR - http://www.scopus.com/inward/record.url?scp=84929907486&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929907486&partnerID=8YFLogxK

U2 - 10.1038/laban.734

DO - 10.1038/laban.734

M3 - Article

VL - 44

SP - 211

EP - 215

JO - Lab Animal

JF - Lab Animal

SN - 0093-7355

IS - 6

ER -