Quantification of amphetamine-induced changes in [11C]raclopride binding with continuous infusion

Richard E. Carson, Alan Breier, Andrea De Bartolomeis, Richard C. Saunders, Tom P. Su, Bernard Schmall, Margaret G. Der, David Pickar, William C. Eckelman

Research output: Contribution to journalArticle

201 Citations (Scopus)

Abstract

Positron emission tomography and single-photon emission computer tomography receptor-binding ligands can be used to measure changes in neurotransmitter levels. In particular, amphetamine-induced dopamine release has been assessed with [11C]raclopride by paired bolus injections and with [123I]iodobenzamide by using a single bolus plus infusion (B/I) study. Here, we measured the change in [11C]raclopride-specific binding in rhesus monkeys after i.v. administration of 0.4 mg/kg amphetamine by using both the bolus and B/I paradigms. Paired bolus studies (control and postamphetamine) were analyzed using compartment modeling and graphical analysis with a new plasma metabolite model to measure the total distribution volume (V(T)). Specific binding, calculated with three measures linearly proportional to the binding potential, demonstrated a 22-42% reduction in the postamphetamine study. V(T) values from B/I studies were determined by the tissue-to-plasma ratio at equilibrium, in addition to the bolus methods. There was good agreement between the control V(T) values between bolus and B/I studies. The amphetamine-induced change in specific binding in B/I studies was 19 ± 16%, measured directly from tissue radioactivity levels. This study demonstrates that stimulus-induced changes in specific binding can be measured with a single [11C]raclopride study using the B/I method.

Original languageEnglish (US)
Pages (from-to)437-447
Number of pages11
JournalJournal of Cerebral Blood Flow and Metabolism
Volume17
Issue number4
StatePublished - 1997
Externally publishedYes

Fingerprint

Raclopride
Amphetamine
Macaca mulatta
Photons
Positron-Emission Tomography
Radioactivity
Neurotransmitter Agents
Dopamine
Tomography
Ligands
Injections

Keywords

  • Amphetamine
  • Infusion
  • Modeling
  • Raclopride

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Carson, R. E., Breier, A., De Bartolomeis, A., Saunders, R. C., Su, T. P., Schmall, B., ... Eckelman, W. C. (1997). Quantification of amphetamine-induced changes in [11C]raclopride binding with continuous infusion. Journal of Cerebral Blood Flow and Metabolism, 17(4), 437-447.

Quantification of amphetamine-induced changes in [11C]raclopride binding with continuous infusion. / Carson, Richard E.; Breier, Alan; De Bartolomeis, Andrea; Saunders, Richard C.; Su, Tom P.; Schmall, Bernard; Der, Margaret G.; Pickar, David; Eckelman, William C.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 17, No. 4, 1997, p. 437-447.

Research output: Contribution to journalArticle

Carson, RE, Breier, A, De Bartolomeis, A, Saunders, RC, Su, TP, Schmall, B, Der, MG, Pickar, D & Eckelman, WC 1997, 'Quantification of amphetamine-induced changes in [11C]raclopride binding with continuous infusion', Journal of Cerebral Blood Flow and Metabolism, vol. 17, no. 4, pp. 437-447.
Carson, Richard E. ; Breier, Alan ; De Bartolomeis, Andrea ; Saunders, Richard C. ; Su, Tom P. ; Schmall, Bernard ; Der, Margaret G. ; Pickar, David ; Eckelman, William C. / Quantification of amphetamine-induced changes in [11C]raclopride binding with continuous infusion. In: Journal of Cerebral Blood Flow and Metabolism. 1997 ; Vol. 17, No. 4. pp. 437-447.
@article{27357b5254e846d88c71e079bc32a17d,
title = "Quantification of amphetamine-induced changes in [11C]raclopride binding with continuous infusion",
abstract = "Positron emission tomography and single-photon emission computer tomography receptor-binding ligands can be used to measure changes in neurotransmitter levels. In particular, amphetamine-induced dopamine release has been assessed with [11C]raclopride by paired bolus injections and with [123I]iodobenzamide by using a single bolus plus infusion (B/I) study. Here, we measured the change in [11C]raclopride-specific binding in rhesus monkeys after i.v. administration of 0.4 mg/kg amphetamine by using both the bolus and B/I paradigms. Paired bolus studies (control and postamphetamine) were analyzed using compartment modeling and graphical analysis with a new plasma metabolite model to measure the total distribution volume (V(T)). Specific binding, calculated with three measures linearly proportional to the binding potential, demonstrated a 22-42{\%} reduction in the postamphetamine study. V(T) values from B/I studies were determined by the tissue-to-plasma ratio at equilibrium, in addition to the bolus methods. There was good agreement between the control V(T) values between bolus and B/I studies. The amphetamine-induced change in specific binding in B/I studies was 19 ± 16{\%}, measured directly from tissue radioactivity levels. This study demonstrates that stimulus-induced changes in specific binding can be measured with a single [11C]raclopride study using the B/I method.",
keywords = "Amphetamine, Infusion, Modeling, Raclopride",
author = "Carson, {Richard E.} and Alan Breier and {De Bartolomeis}, Andrea and Saunders, {Richard C.} and Su, {Tom P.} and Bernard Schmall and Der, {Margaret G.} and David Pickar and Eckelman, {William C.}",
year = "1997",
language = "English (US)",
volume = "17",
pages = "437--447",
journal = "Journal of Cerebral Blood Flow and Metabolism",
issn = "0271-678X",
publisher = "Nature Publishing Group",
number = "4",

}

TY - JOUR

T1 - Quantification of amphetamine-induced changes in [11C]raclopride binding with continuous infusion

AU - Carson, Richard E.

AU - Breier, Alan

AU - De Bartolomeis, Andrea

AU - Saunders, Richard C.

AU - Su, Tom P.

AU - Schmall, Bernard

AU - Der, Margaret G.

AU - Pickar, David

AU - Eckelman, William C.

PY - 1997

Y1 - 1997

N2 - Positron emission tomography and single-photon emission computer tomography receptor-binding ligands can be used to measure changes in neurotransmitter levels. In particular, amphetamine-induced dopamine release has been assessed with [11C]raclopride by paired bolus injections and with [123I]iodobenzamide by using a single bolus plus infusion (B/I) study. Here, we measured the change in [11C]raclopride-specific binding in rhesus monkeys after i.v. administration of 0.4 mg/kg amphetamine by using both the bolus and B/I paradigms. Paired bolus studies (control and postamphetamine) were analyzed using compartment modeling and graphical analysis with a new plasma metabolite model to measure the total distribution volume (V(T)). Specific binding, calculated with three measures linearly proportional to the binding potential, demonstrated a 22-42% reduction in the postamphetamine study. V(T) values from B/I studies were determined by the tissue-to-plasma ratio at equilibrium, in addition to the bolus methods. There was good agreement between the control V(T) values between bolus and B/I studies. The amphetamine-induced change in specific binding in B/I studies was 19 ± 16%, measured directly from tissue radioactivity levels. This study demonstrates that stimulus-induced changes in specific binding can be measured with a single [11C]raclopride study using the B/I method.

AB - Positron emission tomography and single-photon emission computer tomography receptor-binding ligands can be used to measure changes in neurotransmitter levels. In particular, amphetamine-induced dopamine release has been assessed with [11C]raclopride by paired bolus injections and with [123I]iodobenzamide by using a single bolus plus infusion (B/I) study. Here, we measured the change in [11C]raclopride-specific binding in rhesus monkeys after i.v. administration of 0.4 mg/kg amphetamine by using both the bolus and B/I paradigms. Paired bolus studies (control and postamphetamine) were analyzed using compartment modeling and graphical analysis with a new plasma metabolite model to measure the total distribution volume (V(T)). Specific binding, calculated with three measures linearly proportional to the binding potential, demonstrated a 22-42% reduction in the postamphetamine study. V(T) values from B/I studies were determined by the tissue-to-plasma ratio at equilibrium, in addition to the bolus methods. There was good agreement between the control V(T) values between bolus and B/I studies. The amphetamine-induced change in specific binding in B/I studies was 19 ± 16%, measured directly from tissue radioactivity levels. This study demonstrates that stimulus-induced changes in specific binding can be measured with a single [11C]raclopride study using the B/I method.

KW - Amphetamine

KW - Infusion

KW - Modeling

KW - Raclopride

UR - http://www.scopus.com/inward/record.url?scp=0030888681&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030888681&partnerID=8YFLogxK

M3 - Article

C2 - 9143226

AN - SCOPUS:0030888681

VL - 17

SP - 437

EP - 447

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

SN - 0271-678X

IS - 4

ER -