Quantification of P53 in oral epithelial dysplasias via confocal microscopy vs standard microscopy

S. Ganatra, D. J. Summerlin, A. H. Kafrawy, C. E. Tomich, M. L. Van Dis, Susan Zunt

Research output: Contribution to journalArticle

Abstract

p53 expression has been shown to be elevated in oral epithelial dysplasias associated with alcohol and tobacco use. The intent of this investigation was twofold: to determine if this association was reproducible and to determine if immunofluorescent testing with confocal microscopy represented a more sensitive and objective analytic tool than standard immunohistochemistry. Sixty-one cases of epithelial dysplasia were obtained from the Indiana University Oral Pathology Biopsy Service and divided into three groups. Group 1 consisted of 19 dysplasias from patients with no habits. Group 2 consisted of 21 dysplasias from patients with a history of smoking without alcohol use. Group 3 consisted of 21 dysplasias from patients with a history of smoking and alcohol consumption. Each case underwent standard immunohistochemical testing with subjective analysis as well as immunofluorescent testing with computer quantification by a confocal laser microscope and associated software. Both techniques were used to quantify intensity of stain and percentage of positive cells (percent area). Immunohistochemical analysis of percent area and stain intensity were not significantly different between the groups. Immunofluorescent analysis, however, did demonstrate significantly higher intensity (p <0.03) for group 3 and group 1 than for group 2 but percent area measurements for the three groups were not significantly different. Intensity measurements between the two methods were not correlated (p = 0.22) but percent area measurements were (p <0.01). This study differs from previous studies in that no difference was noted in p53 expression in dysplasia with or without habits. In addition, immunofluorescent testing with confocal microscopy was found to produce significantly different values of percent area and represents a more objective mechanism of analysis.

Original languageEnglish (US)
Pages (from-to)211
Number of pages1
JournalOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
Volume82
Issue number2
StatePublished - 1996

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Confocal Microscopy
Microscopy
Habits
Coloring Agents
Smoking
Alcohols
Oral Pathology
Tobacco Use
Alcohol Drinking
Lasers
Software
Immunohistochemistry
Biopsy

ASJC Scopus subject areas

  • Dentistry(all)
  • Pathology and Forensic Medicine
  • Radiology Nuclear Medicine and imaging
  • Surgery

Cite this

Quantification of P53 in oral epithelial dysplasias via confocal microscopy vs standard microscopy. / Ganatra, S.; Summerlin, D. J.; Kafrawy, A. H.; Tomich, C. E.; Van Dis, M. L.; Zunt, Susan.

In: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, Vol. 82, No. 2, 1996, p. 211.

Research output: Contribution to journalArticle

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