Quantification of tamoxifen and three metabolites in plasma by high-performance liquid chromatography with fluorescence detection: Application to a clinical trial

Kyung Hoon Lee, Bryan A. Ward, Zeruesenay Desta, David A. Flockhart, David R. Jones

Research output: Contribution to journalArticle

84 Scopus citations

Abstract

A sensitive and reproducible assay employing liquid-liquid extraction and high-performance liquid chromatography with fluorescence detection for the quantification of tamoxifen, N-desmethyltamoxifen, 4-hydroxytamoxifen, and Z-4-hydroxy-N-desmethyltamoxifen in human plasma is described. The compounds and internal standard, propranolol, were separated with a cyano column and a mobile phase of acetonitrile-20 mM potassium phosphate buffer (pH 3; 35:65, v/v) then detected with fluorescence using a modified version of a method originally described by Fried and Wainer [J. Chromatogr. B 655 (1994) 261]. The coefficients of variation for the midpoint of the standard curve for each compound were less than 10%. This method was applied to a pharmacokinetic study of tamoxifen disposition in breast cancer patients.

Original languageEnglish (US)
Pages (from-to)245-253
Number of pages9
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume791
Issue number1-2
DOIs
StatePublished - Jul 5 2003

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Keywords

  • 4-Hydroxytamoxifen
  • N-Desmethyltamoxifen
  • Tamoxifen
  • Z-4-Hydroxy-N-desmethyltamoxifen

ASJC Scopus subject areas

  • Biochemistry

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