Quantitation of proton NMR spectra of the human brain using tissue water as an internal concentration reference.

P. B. Barker, B. J. Soher, S. J. Blackband, J. C. Chatham, Vincent Mathews, R. N. Bryan

Research output: Contribution to journalArticle

251 Citations (Scopus)

Abstract

The use of cerebral water as an internal intensity standard for the quantitation of spatially localized proton spectra of the human brain is investigated. The method is validated on standard samples of N-acetyl aspartate (NAA) and lactate, and possible sources of error are discussed. Using the STEAM pulse sequence, concentrations of choline, creatine and NAA in frontal lobe white matter are found to be 1.9 +/- 0.5, 10.6 +/- 1.3 and 16.6 +/- 2.3 mumol/g wet wt, respectively, in 10 normal volunteers. In the thalamus, the concentrations are 2.0 +/- 0.4, 11.6 +/- 2.0 and 17.2 +/- 1.3 mumol/g wet wt, respectively. Choline and creatine concentrations are in good agreement with conventional biochemical values: NAA concentrations are found to be three-fold higher, suggesting overlap of the NAA signal with other compounds. Quantitation relative to tissue water is a convenient and rapid means of quantitating proton spectra of the human brain.

Original languageEnglish (US)
Pages (from-to)89-94
Number of pages6
JournalNMR in Biomedicine
Volume6
Issue number1
StatePublished - Jan 1993
Externally publishedYes

Fingerprint

Protons
Brain
Nuclear magnetic resonance
Tissue
Creatine
Water
Choline
Frontal Lobe
Thalamus
Lactic Acid
Healthy Volunteers
Research Design
N-acetylaspartate

ASJC Scopus subject areas

  • Biophysics
  • Spectroscopy
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Barker, P. B., Soher, B. J., Blackband, S. J., Chatham, J. C., Mathews, V., & Bryan, R. N. (1993). Quantitation of proton NMR spectra of the human brain using tissue water as an internal concentration reference. NMR in Biomedicine, 6(1), 89-94.

Quantitation of proton NMR spectra of the human brain using tissue water as an internal concentration reference. / Barker, P. B.; Soher, B. J.; Blackband, S. J.; Chatham, J. C.; Mathews, Vincent; Bryan, R. N.

In: NMR in Biomedicine, Vol. 6, No. 1, 01.1993, p. 89-94.

Research output: Contribution to journalArticle

Barker, PB, Soher, BJ, Blackband, SJ, Chatham, JC, Mathews, V & Bryan, RN 1993, 'Quantitation of proton NMR spectra of the human brain using tissue water as an internal concentration reference.', NMR in Biomedicine, vol. 6, no. 1, pp. 89-94.
Barker, P. B. ; Soher, B. J. ; Blackband, S. J. ; Chatham, J. C. ; Mathews, Vincent ; Bryan, R. N. / Quantitation of proton NMR spectra of the human brain using tissue water as an internal concentration reference. In: NMR in Biomedicine. 1993 ; Vol. 6, No. 1. pp. 89-94.
@article{ef2f53ce0a5144c5a0a1b3178f70fe04,
title = "Quantitation of proton NMR spectra of the human brain using tissue water as an internal concentration reference.",
abstract = "The use of cerebral water as an internal intensity standard for the quantitation of spatially localized proton spectra of the human brain is investigated. The method is validated on standard samples of N-acetyl aspartate (NAA) and lactate, and possible sources of error are discussed. Using the STEAM pulse sequence, concentrations of choline, creatine and NAA in frontal lobe white matter are found to be 1.9 +/- 0.5, 10.6 +/- 1.3 and 16.6 +/- 2.3 mumol/g wet wt, respectively, in 10 normal volunteers. In the thalamus, the concentrations are 2.0 +/- 0.4, 11.6 +/- 2.0 and 17.2 +/- 1.3 mumol/g wet wt, respectively. Choline and creatine concentrations are in good agreement with conventional biochemical values: NAA concentrations are found to be three-fold higher, suggesting overlap of the NAA signal with other compounds. Quantitation relative to tissue water is a convenient and rapid means of quantitating proton spectra of the human brain.",
author = "Barker, {P. B.} and Soher, {B. J.} and Blackband, {S. J.} and Chatham, {J. C.} and Vincent Mathews and Bryan, {R. N.}",
year = "1993",
month = "1",
language = "English (US)",
volume = "6",
pages = "89--94",
journal = "NMR in Biomedicine",
issn = "0952-3480",
publisher = "John Wiley and Sons Ltd",
number = "1",

}

TY - JOUR

T1 - Quantitation of proton NMR spectra of the human brain using tissue water as an internal concentration reference.

AU - Barker, P. B.

AU - Soher, B. J.

AU - Blackband, S. J.

AU - Chatham, J. C.

AU - Mathews, Vincent

AU - Bryan, R. N.

PY - 1993/1

Y1 - 1993/1

N2 - The use of cerebral water as an internal intensity standard for the quantitation of spatially localized proton spectra of the human brain is investigated. The method is validated on standard samples of N-acetyl aspartate (NAA) and lactate, and possible sources of error are discussed. Using the STEAM pulse sequence, concentrations of choline, creatine and NAA in frontal lobe white matter are found to be 1.9 +/- 0.5, 10.6 +/- 1.3 and 16.6 +/- 2.3 mumol/g wet wt, respectively, in 10 normal volunteers. In the thalamus, the concentrations are 2.0 +/- 0.4, 11.6 +/- 2.0 and 17.2 +/- 1.3 mumol/g wet wt, respectively. Choline and creatine concentrations are in good agreement with conventional biochemical values: NAA concentrations are found to be three-fold higher, suggesting overlap of the NAA signal with other compounds. Quantitation relative to tissue water is a convenient and rapid means of quantitating proton spectra of the human brain.

AB - The use of cerebral water as an internal intensity standard for the quantitation of spatially localized proton spectra of the human brain is investigated. The method is validated on standard samples of N-acetyl aspartate (NAA) and lactate, and possible sources of error are discussed. Using the STEAM pulse sequence, concentrations of choline, creatine and NAA in frontal lobe white matter are found to be 1.9 +/- 0.5, 10.6 +/- 1.3 and 16.6 +/- 2.3 mumol/g wet wt, respectively, in 10 normal volunteers. In the thalamus, the concentrations are 2.0 +/- 0.4, 11.6 +/- 2.0 and 17.2 +/- 1.3 mumol/g wet wt, respectively. Choline and creatine concentrations are in good agreement with conventional biochemical values: NAA concentrations are found to be three-fold higher, suggesting overlap of the NAA signal with other compounds. Quantitation relative to tissue water is a convenient and rapid means of quantitating proton spectra of the human brain.

UR - http://www.scopus.com/inward/record.url?scp=0027345896&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027345896&partnerID=8YFLogxK

M3 - Article

C2 - 8384470

AN - SCOPUS:0027345896

VL - 6

SP - 89

EP - 94

JO - NMR in Biomedicine

JF - NMR in Biomedicine

SN - 0952-3480

IS - 1

ER -