Quantitation of shock wave cavitation damage in vitro

David A. Lifshitz, James Williams, Bradford Sturtevant, Bret A. Connors, Andrew Evan, James A. Mcateer

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Acoustic cavitation damage was quantitated using aluminum foil targets placed within 2-mL polypropylene cryovials. The vials contained various media tested for their potential to support cavitation and were exposed to shock waves using an unmodified Dornier HM3 lithotripter. Foil damage, expressed in terms of a 'damage index,' was measured from digitized light microscopy images by quantitating the spread of gray-scale histograms. Target sensitivity was demonstrated by reproducible dose-response curves over the range (1-200 shock waves) commonly used for in vitro cell injury studies. Increased shock wave repetition rate reduced the damage index. Untreated foils showed a very low damage index (0.001% ± 0.001%), while treated foils submerged in Ringer buffer yielded significant damage (2.2% ± 0.3%, p < 0.001). Degassing the buffer reduced damage to 0.3% ± 0.1% (p < 0.001). Foils submerged in castor oil showed virtually no damage. These results implicate acoustic cavitation in target damage. Targets immersed in biological fluids (blood and urine) had significantly less damage than in Ringer. The effect of degassing was also evaluated in a red blood cell lysis assay. Hemoglobin release in degassed preparations was significantly reduced compared to nondegassed controls (p < 0.001) and correlated with reduced foil damage index in cell-free vials. These findings characterize a sensitive method to quantitate acoustic cavitation and implicate a role for cavitation in shock wave lithotripsy-induced cell lysis.

Original languageEnglish
Pages (from-to)461-471
Number of pages11
JournalUltrasound in Medicine and Biology
Volume23
Issue number3
DOIs
StatePublished - 1997

Fingerprint

cavitation flow
Acoustics
shock waves
damage
Buffers
Castor Oil
foils
Lithotripsy
Polypropylenes
Aluminum
Microscopy
Hemoglobins
Erythrocytes
Urine
Light
degassing
Wounds and Injuries
acoustics
In Vitro Techniques
buffers

Keywords

  • Acoustic cavitation
  • Cell lysis
  • Image analysis
  • Red blood cells
  • Shock wave lithotripsy
  • Shock waves

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Quantitation of shock wave cavitation damage in vitro. / Lifshitz, David A.; Williams, James; Sturtevant, Bradford; Connors, Bret A.; Evan, Andrew; Mcateer, James A.

In: Ultrasound in Medicine and Biology, Vol. 23, No. 3, 1997, p. 461-471.

Research output: Contribution to journalArticle

Lifshitz, David A. ; Williams, James ; Sturtevant, Bradford ; Connors, Bret A. ; Evan, Andrew ; Mcateer, James A. / Quantitation of shock wave cavitation damage in vitro. In: Ultrasound in Medicine and Biology. 1997 ; Vol. 23, No. 3. pp. 461-471.
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