Quantitative autoradiography of mu-opioid receptors in the CNS of alcohol-naive alcohol-preferring P and -nonpreferring NP rats

W. J. McBride, E. Chernet, D. L. McKinzie, L. Lumeng, T. K. Li

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The densities of mu-opioid binding sites in the CNS of alcohol-naive adult male P and NP rats (N = 9 each line) were examined using quantitative autoradiography. Coronal sections (20 μm) were prepared from frozen brains and incubated in 5 nM [3H]DAMGO to label mu-opioid receptor sites. Nonspecific binding was determined in the presence of 1μM DAMGO. The amount of [3H]DAMGO binding was (a) 20-25% higher in the olfactory tubercle, nucleus accumbens shell and core, and basolateral and lateral amygdaloid nuclei; (b) 15% higher in the lateral septal intermediate nucleus and caudate-putamen patches; and (c) 10-30% lower in the pyramidal and radiatum layers in the CA1 region of the anterior dorsal hippocampus, ventral dentate gyrus and CA1 pyramidal layer of the posterior hippocampus, and posterior medial cortical amygdaloid nucleus of the P compared to the NP rat. No line differences were found in any of the other regions examined (e.g., the cerebral cortical subregions and layers, thalamic nuclei, ventral tegmental area, ventral pallidum, lateral hypothalamus, other regions of the hippocampus, and several subcortical structures). The innate differences in the amount of binding to mu-opioid recognition sites in certain limbic structures, such as the nucleus accumbens, amygdala, and olfactory tubercle, of the P and NP lines may be factors contributing to their disparate alcohol drinking characteristics.

Original languageEnglish
Pages (from-to)317-323
Number of pages7
JournalAlcohol
Volume16
Issue number4
DOIs
StatePublished - Nov 1998

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Ala(2)-MePhe(4)-Gly(5)-enkephalin
mu Opioid Receptor
Autoradiography
Rats
Hippocampus
alcohol
Alcohols
Nucleus Accumbens
Opioid Analgesics
Lateral Hypothalamic Area
Thalamic Nuclei
Septal Nuclei
Ventral Tegmental Area
Putamen
Dentate Gyrus
Amygdala
Alcohol Drinking
Labels
Brain
brain

Keywords

  • Alcohol-preferring rats DAMGO
  • Mu-opioid receptor
  • Quantitative autoradiography

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Behavioral Neuroscience
  • Neuroscience(all)
  • Toxicology
  • Health(social science)

Cite this

Quantitative autoradiography of mu-opioid receptors in the CNS of alcohol-naive alcohol-preferring P and -nonpreferring NP rats. / McBride, W. J.; Chernet, E.; McKinzie, D. L.; Lumeng, L.; Li, T. K.

In: Alcohol, Vol. 16, No. 4, 11.1998, p. 317-323.

Research output: Contribution to journalArticle

McBride, W. J. ; Chernet, E. ; McKinzie, D. L. ; Lumeng, L. ; Li, T. K. / Quantitative autoradiography of mu-opioid receptors in the CNS of alcohol-naive alcohol-preferring P and -nonpreferring NP rats. In: Alcohol. 1998 ; Vol. 16, No. 4. pp. 317-323.
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