Quantitative chiral analysis of salsolinol in different brain regions of rats genetically predisposed to alcoholism

Tatiana Rojkovicova, Yehia Mechref, Jason A. Starkey, Guangxiang Wu, Richard Bell, William J. McBride, Milos V. Novotny

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

A method to determine the catecholamine content in putamen (CPU) and midbrain (MB) regions of the brain of alcohol-preferring rats (P) is presented with a focus on the low-level detection of S,R-salsolinol, a metabolite of dopamine and a putative alcoholism marker. The developed strategy allows both quantitative profiling of related catecholamines and the enantiomeric separation and quantification of the S- and R-salsolinol isomers and their ratios. The described LC/MS strategy simplifies the current methodology that typically employs GC-MS by eliminating the need for derivatization. The data also suggest an increase in the non-enzymatic formation of salsolinol as a consequence of ethanol exposure.

Original languageEnglish
Pages (from-to)206-214
Number of pages9
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume863
Issue number2
DOIs
StatePublished - Mar 1 2008

Fingerprint

Alcoholism
Rats
Brain
Catecholamines
Chemical analysis
Putamen
Metabolites
Mesencephalon
Isomers
Dopamine
Ethanol
Alcohols
salsolinol

Keywords

  • Alcoholism
  • Catecholamines
  • Chiral separations
  • Multiple reaction monitoring (MRM)
  • Salsolinol
  • Tandem mass spectrometry

ASJC Scopus subject areas

  • Biochemistry

Cite this

Quantitative chiral analysis of salsolinol in different brain regions of rats genetically predisposed to alcoholism. / Rojkovicova, Tatiana; Mechref, Yehia; Starkey, Jason A.; Wu, Guangxiang; Bell, Richard; McBride, William J.; Novotny, Milos V.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 863, No. 2, 01.03.2008, p. 206-214.

Research output: Contribution to journalArticle

Rojkovicova, Tatiana ; Mechref, Yehia ; Starkey, Jason A. ; Wu, Guangxiang ; Bell, Richard ; McBride, William J. ; Novotny, Milos V. / Quantitative chiral analysis of salsolinol in different brain regions of rats genetically predisposed to alcoholism. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2008 ; Vol. 863, No. 2. pp. 206-214.
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