Quantitative comparison of mu opioid receptor mRNA in selected CNS regions of alcohol naive rats selectively bred for high and low alcohol drinking

Jianhua Gong, Xuan Wen Li, Zhennan Lai, Janice C. Froehlich, Lei Yu

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We combined solution hybridization, ribonuclease protection and microdissection techniques to quantitatively compare the anatomical distribution of mu receptor mRNA in discrete brain regions of alcohol naive rats selectively bred for high and low alcohol drinking (HAD and LAD lines, respectively). The solution hybridization assay is highly sensitive and can detect mu opioid receptor mRNA in a 100-fold linear range from 4 to 421 amol. HAD and LAD rats exhibited a similar level of mu receptor mRNA in all central nervous system (CNS) regions examined except for the inferior colliculus. Our data suggest that the steady-state level of mu receptor mRNA is not associated with genetic differences in alcohol drinking behavior.

Original languageEnglish (US)
Pages (from-to)9-12
Number of pages4
JournalNeuroscience Letters
Volume227
Issue number1
DOIs
StatePublished - May 9 1997

Keywords

  • Alcohol drinking
  • Mu opioid receptor
  • Solution hybridization

ASJC Scopus subject areas

  • Neuroscience(all)

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