Quantitative estimation of PCNA, c-myc, EGFR and TGF-α in oral submucous fibrosis - An immunohistochemical study

M. Srinivasan, S. D. Jewell

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

The objectives of the present study were to evaluate the expression of three proliferation markers, viz., epidermal growth factor receptor (EGFR), transforming growth factor-alpha (TGF-α) and proliferating cell nuclear antigen (PCNA) and one genomic marker, c-myc in OSMF. Oral tissues were stained with monoclonal antibodies to PCNA, c-myc, TGF-α and EGFR. The results were analyzed with Photoquant image analysis software. The expression of PCNA, c-myc, TGF-α and EGFR was higher in oral submucous fibrosis (OSMF) than in normal control oral mucosa. The oral epithelium was divided into a proliferative compartment (stratum germinativum) and a differentiated compartment (stratum spinosum). A differential pattern of expression of PCNA and c-myc was observed in OSMF. While the intensity of staining decreased, the percent area of expression of PCNA and c-myc increased in stratum germinativum in OSMF (P<0.05). This suggests that greater proportions of cells exhibit PCNA and c-myc immunoreactivity and are in the proliferative pool in OSMF. TGF-α levels increased in the proliferative layers and EGFR levels increased in the differentiated layers (P<0.05) of the oral epithelium in OSMF. Quantitative measurement of these oncoproteins substantiates the precancerous nature of OSMF and may provide intermediate end-points in prospective chemopreventive trials.

Original languageEnglish (US)
Pages (from-to)461-467
Number of pages7
JournalOral Oncology
Volume37
Issue number5
DOIs
StatePublished - Jul 1 2001
Externally publishedYes

Keywords

  • EGFR
  • Oral submucous fibrosis
  • PCNA
  • Quantitative image analysis
  • TGF-α
  • c-myc

ASJC Scopus subject areas

  • Oral Surgery
  • Oncology
  • Cancer Research

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