Quantitative estimation of PCNA, c-myc, EGFR and TGF-α in oral submucous fibrosis - An immunohistochemical study

M. Srinivasan, S. D. Jewell

Research output: Contribution to journalArticle

30 Scopus citations


The objectives of the present study were to evaluate the expression of three proliferation markers, viz., epidermal growth factor receptor (EGFR), transforming growth factor-alpha (TGF-α) and proliferating cell nuclear antigen (PCNA) and one genomic marker, c-myc in OSMF. Oral tissues were stained with monoclonal antibodies to PCNA, c-myc, TGF-α and EGFR. The results were analyzed with Photoquant image analysis software. The expression of PCNA, c-myc, TGF-α and EGFR was higher in oral submucous fibrosis (OSMF) than in normal control oral mucosa. The oral epithelium was divided into a proliferative compartment (stratum germinativum) and a differentiated compartment (stratum spinosum). A differential pattern of expression of PCNA and c-myc was observed in OSMF. While the intensity of staining decreased, the percent area of expression of PCNA and c-myc increased in stratum germinativum in OSMF (P<0.05). This suggests that greater proportions of cells exhibit PCNA and c-myc immunoreactivity and are in the proliferative pool in OSMF. TGF-α levels increased in the proliferative layers and EGFR levels increased in the differentiated layers (P<0.05) of the oral epithelium in OSMF. Quantitative measurement of these oncoproteins substantiates the precancerous nature of OSMF and may provide intermediate end-points in prospective chemopreventive trials.

Original languageEnglish (US)
Pages (from-to)461-467
Number of pages7
JournalOral Oncology
Issue number5
StatePublished - Jul 1 2001
Externally publishedYes


  • EGFR
  • Oral submucous fibrosis
  • PCNA
  • Quantitative image analysis
  • TGF-α
  • c-myc

ASJC Scopus subject areas

  • Oral Surgery
  • Oncology
  • Cancer Research

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