Quantitative estimation of PCNA, c-myc, EGFR and TGF-α in oral submucous fibrosis - An immunohistochemical study

Mythily Srinivasan, S. D. Jewell

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The objectives of the present study were to evaluate the expression of three proliferation markers, viz., epidermal growth factor receptor (EGFR), transforming growth factor-alpha (TGF-α) and proliferating cell nuclear antigen (PCNA) and one genomic marker, c-myc in OSMF. Oral tissues were stained with monoclonal antibodies to PCNA, c-myc, TGF-α and EGFR. The results were analyzed with Photoquant image analysis software. The expression of PCNA, c-myc, TGF-α and EGFR was higher in oral submucous fibrosis (OSMF) than in normal control oral mucosa. The oral epithelium was divided into a proliferative compartment (stratum germinativum) and a differentiated compartment (stratum spinosum). A differential pattern of expression of PCNA and c-myc was observed in OSMF. While the intensity of staining decreased, the percent area of expression of PCNA and c-myc increased in stratum germinativum in OSMF (P

Original languageEnglish (US)
Pages (from-to)461-467
Number of pages7
JournalOral Oncology
Volume37
Issue number5
DOIs
StatePublished - Jul 2001
Externally publishedYes

Fingerprint

Oral Submucous Fibrosis
Transforming Growth Factor alpha
Proliferating Cell Nuclear Antigen
Epidermal Growth Factor Receptor
Mouth Mucosa
Software
Epithelium
Monoclonal Antibodies
Staining and Labeling

Keywords

  • c-myc
  • EGFR
  • Oral submucous fibrosis
  • PCNA
  • Quantitative image analysis
  • TGF-α

ASJC Scopus subject areas

  • Oncology

Cite this

Quantitative estimation of PCNA, c-myc, EGFR and TGF-α in oral submucous fibrosis - An immunohistochemical study. / Srinivasan, Mythily; Jewell, S. D.

In: Oral Oncology, Vol. 37, No. 5, 07.2001, p. 461-467.

Research output: Contribution to journalArticle

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