Quantitative quality-assessment techniques to compare fractionation and depletion methods in SELDI-TOF mass spectrometry experiments

Jaroslaw Harezlak, Mike Wang, David Christiani, Xihong Lin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Motivation: Mass spectrometry (MS), such as the surface-enhanced laser desorption and ionization time-of-flight (SELDI-TOF) MS, provides a potentially promising proteomic technology for biomarker discovery. An important matter for such a technology to be used routinely is its reproducibility. It is of significant interest to develop quantitative measures to evaluate the quality and reliability of different experimental methods. Results: We compare the quality of SELDI-TOF MS data using unfractionated, fractionated plasma samples and abundant protein depletion methods in terms of the numbers of detected peaks and reliability. Several statistical quality-control and quality-assessment techniques are proposed, including the Graeco-Latin square design for the sample allocation on a Protein chip, the use of the pairwise Pearson correlation coefficient as the similarity measure between the spectra in conjunction with multi-dimensional scaling (MDS) for graphically evaluating similarity of replicates and assessing outlier samples; and the use of the reliability ratio for evaluating reproducibility. Our results show that the number of peaks detected is similar among the three sample preparation technologies, and the use of the Sigma multi-removal kit does not improve peak detection. Fractionation of plasma samples introduces more experimental variability. The peaks detected using the unfractionated plasma samples have the highest reproducibility as determined by the reliability ratio.

Original languageEnglish (US)
Pages (from-to)2441-2448
Number of pages8
JournalBioinformatics
Volume23
Issue number18
DOIs
StatePublished - Sep 15 2007
Externally publishedYes

Fingerprint

Desorption
Quality Assessment
Time-of-flight
Mass Spectrometry
Fractionation
Depletion
Ionization
Mass spectrometry
Lasers
Laser
Technology
Reproducibility
Plasmas
Experiment
Protein Array Analysis
Plasma
Experiments
Proteins
Quality Control
Proteomics

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Computer Science Applications
  • Computational Theory and Mathematics

Cite this

Quantitative quality-assessment techniques to compare fractionation and depletion methods in SELDI-TOF mass spectrometry experiments. / Harezlak, Jaroslaw; Wang, Mike; Christiani, David; Lin, Xihong.

In: Bioinformatics, Vol. 23, No. 18, 15.09.2007, p. 2441-2448.

Research output: Contribution to journalArticle

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