Quantitative trait loci (QTL) for circadian rhythms of locomotor activity in mice

John R. Hofstetter, Aimee R. Mayeda, Bernard Possidente, John I. Nurnberger

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The loomotor activity of male mice (Mus musculus) was monitored by infrared photoelectric beams under three lighting regimens: LD (12 h of light and 12 h of dark), DD (constant dark), and LL (constant broad-spectrum light, 10 lux). Circadian period of locomotor activioty (τ) was compared among 3 inbred strains of mice, C57BL/6J (B6), BALB/c (C), and DBA/2J (D2), and 26 recombinant inbred strains B×D (B6×D2). the τ under both continuous low-intensity light and continuous darkenss varied significantly among strains. Under DD the mean τ was 23.8 h for B6, 23.7 h for D2, and 23.6 h for C. Under LL the mean τ was 25.1 for B6, 23.9 h for D2, and 25.5 h for C. Frequency histograms of the mean τ of 26B×D RI mouse strains (three to seven animals per strain) in either DD or LL and the difference between them, Δτ, had distributions which appeared unimodal, suggesting polygenic inheritances. The narrow-sense heritability determined using 26 strains of B×D RI mice was about 55% for τ and about 38% for both τ in LL and Δτ. An estimated four loci contribute to the variance of τ in constant darkness and five to the variance of τ in constant low-intensity light among the strains studied. Quantitative trait locus (QTL) analysis identified several potential genetic loci associated with τ in constant darkness, τ in constant low-intensity light, and Δτ. The associations of highest probability for each of these traits were the D1Nds4 locus (p<0.001) on mouse chromosome 1, the D5Ncvs52 locus (p<.05) on mouse chromosome 5, and the Pmv12 locus (p<.01) at 70 cM on mouse chromosome 5, respectively. A QTL identified for τ was associated (p<.05) with the D2NDS1 marker at 45 cM on chromsome 2 near the Ea 6 marker at 46 cM associated (p<.05) with that reported for the period of wheel running activity in seven C×B RI strains (Schwartz, W. J., and Zimmerman, P., J. Neurosci.10:3685 1990).

Original languageEnglish (US)
Pages (from-to)545-556
Number of pages12
JournalBehavior Genetics
Volume25
Issue number6
DOIs
StatePublished - Nov 1 1995

Fingerprint

circadian rhythm
Quantitative Trait Loci
Locomotion
Circadian Rhythm
locomotion
quantitative trait loci
light intensity
chromosome
mice
Light
loci
Chromosomes, Human, Pair 5
Darkness
heritability
histogram
chromosomes
Multifactorial Inheritance
polygenic inheritance
Inbred Strains Mice
Genetic Loci

Keywords

  • circadian rhythms
  • genetics
  • light
  • locomotor activity
  • quantitative trait loci
  • Recombinant inbred mouse

ASJC Scopus subject areas

  • Psychology(all)
  • Behavioral Neuroscience
  • Genetics(clinical)
  • Genetics

Cite this

Quantitative trait loci (QTL) for circadian rhythms of locomotor activity in mice. / Hofstetter, John R.; Mayeda, Aimee R.; Possidente, Bernard; Nurnberger, John I.

In: Behavior Genetics, Vol. 25, No. 6, 01.11.1995, p. 545-556.

Research output: Contribution to journalArticle

Hofstetter, John R. ; Mayeda, Aimee R. ; Possidente, Bernard ; Nurnberger, John I. / Quantitative trait loci (QTL) for circadian rhythms of locomotor activity in mice. In: Behavior Genetics. 1995 ; Vol. 25, No. 6. pp. 545-556.
@article{b0728dc35f2a42c196c9b1bbbd4c2974,
title = "Quantitative trait loci (QTL) for circadian rhythms of locomotor activity in mice",
abstract = "The loomotor activity of male mice (Mus musculus) was monitored by infrared photoelectric beams under three lighting regimens: LD (12 h of light and 12 h of dark), DD (constant dark), and LL (constant broad-spectrum light, 10 lux). Circadian period of locomotor activioty (τ) was compared among 3 inbred strains of mice, C57BL/6J (B6), BALB/c (C), and DBA/2J (D2), and 26 recombinant inbred strains B×D (B6×D2). the τ under both continuous low-intensity light and continuous darkenss varied significantly among strains. Under DD the mean τ was 23.8 h for B6, 23.7 h for D2, and 23.6 h for C. Under LL the mean τ was 25.1 for B6, 23.9 h for D2, and 25.5 h for C. Frequency histograms of the mean τ of 26B×D RI mouse strains (three to seven animals per strain) in either DD or LL and the difference between them, Δτ, had distributions which appeared unimodal, suggesting polygenic inheritances. The narrow-sense heritability determined using 26 strains of B×D RI mice was about 55{\%} for τ and about 38{\%} for both τ in LL and Δτ. An estimated four loci contribute to the variance of τ in constant darkness and five to the variance of τ in constant low-intensity light among the strains studied. Quantitative trait locus (QTL) analysis identified several potential genetic loci associated with τ in constant darkness, τ in constant low-intensity light, and Δτ. The associations of highest probability for each of these traits were the D1Nds4 locus (p<0.001) on mouse chromosome 1, the D5Ncvs52 locus (p<.05) on mouse chromosome 5, and the Pmv12 locus (p<.01) at 70 cM on mouse chromosome 5, respectively. A QTL identified for τ was associated (p<.05) with the D2NDS1 marker at 45 cM on chromsome 2 near the Ea 6 marker at 46 cM associated (p<.05) with that reported for the period of wheel running activity in seven C×B RI strains (Schwartz, W. J., and Zimmerman, P., J. Neurosci.10:3685 1990).",
keywords = "circadian rhythms, genetics, light, locomotor activity, quantitative trait loci, Recombinant inbred mouse",
author = "Hofstetter, {John R.} and Mayeda, {Aimee R.} and Bernard Possidente and Nurnberger, {John I.}",
year = "1995",
month = "11",
day = "1",
doi = "10.1007/BF02327578",
language = "English (US)",
volume = "25",
pages = "545--556",
journal = "Behavior Genetics",
issn = "0001-8244",
publisher = "Springer New York",
number = "6",

}

TY - JOUR

T1 - Quantitative trait loci (QTL) for circadian rhythms of locomotor activity in mice

AU - Hofstetter, John R.

AU - Mayeda, Aimee R.

AU - Possidente, Bernard

AU - Nurnberger, John I.

PY - 1995/11/1

Y1 - 1995/11/1

N2 - The loomotor activity of male mice (Mus musculus) was monitored by infrared photoelectric beams under three lighting regimens: LD (12 h of light and 12 h of dark), DD (constant dark), and LL (constant broad-spectrum light, 10 lux). Circadian period of locomotor activioty (τ) was compared among 3 inbred strains of mice, C57BL/6J (B6), BALB/c (C), and DBA/2J (D2), and 26 recombinant inbred strains B×D (B6×D2). the τ under both continuous low-intensity light and continuous darkenss varied significantly among strains. Under DD the mean τ was 23.8 h for B6, 23.7 h for D2, and 23.6 h for C. Under LL the mean τ was 25.1 for B6, 23.9 h for D2, and 25.5 h for C. Frequency histograms of the mean τ of 26B×D RI mouse strains (three to seven animals per strain) in either DD or LL and the difference between them, Δτ, had distributions which appeared unimodal, suggesting polygenic inheritances. The narrow-sense heritability determined using 26 strains of B×D RI mice was about 55% for τ and about 38% for both τ in LL and Δτ. An estimated four loci contribute to the variance of τ in constant darkness and five to the variance of τ in constant low-intensity light among the strains studied. Quantitative trait locus (QTL) analysis identified several potential genetic loci associated with τ in constant darkness, τ in constant low-intensity light, and Δτ. The associations of highest probability for each of these traits were the D1Nds4 locus (p<0.001) on mouse chromosome 1, the D5Ncvs52 locus (p<.05) on mouse chromosome 5, and the Pmv12 locus (p<.01) at 70 cM on mouse chromosome 5, respectively. A QTL identified for τ was associated (p<.05) with the D2NDS1 marker at 45 cM on chromsome 2 near the Ea 6 marker at 46 cM associated (p<.05) with that reported for the period of wheel running activity in seven C×B RI strains (Schwartz, W. J., and Zimmerman, P., J. Neurosci.10:3685 1990).

AB - The loomotor activity of male mice (Mus musculus) was monitored by infrared photoelectric beams under three lighting regimens: LD (12 h of light and 12 h of dark), DD (constant dark), and LL (constant broad-spectrum light, 10 lux). Circadian period of locomotor activioty (τ) was compared among 3 inbred strains of mice, C57BL/6J (B6), BALB/c (C), and DBA/2J (D2), and 26 recombinant inbred strains B×D (B6×D2). the τ under both continuous low-intensity light and continuous darkenss varied significantly among strains. Under DD the mean τ was 23.8 h for B6, 23.7 h for D2, and 23.6 h for C. Under LL the mean τ was 25.1 for B6, 23.9 h for D2, and 25.5 h for C. Frequency histograms of the mean τ of 26B×D RI mouse strains (three to seven animals per strain) in either DD or LL and the difference between them, Δτ, had distributions which appeared unimodal, suggesting polygenic inheritances. The narrow-sense heritability determined using 26 strains of B×D RI mice was about 55% for τ and about 38% for both τ in LL and Δτ. An estimated four loci contribute to the variance of τ in constant darkness and five to the variance of τ in constant low-intensity light among the strains studied. Quantitative trait locus (QTL) analysis identified several potential genetic loci associated with τ in constant darkness, τ in constant low-intensity light, and Δτ. The associations of highest probability for each of these traits were the D1Nds4 locus (p<0.001) on mouse chromosome 1, the D5Ncvs52 locus (p<.05) on mouse chromosome 5, and the Pmv12 locus (p<.01) at 70 cM on mouse chromosome 5, respectively. A QTL identified for τ was associated (p<.05) with the D2NDS1 marker at 45 cM on chromsome 2 near the Ea 6 marker at 46 cM associated (p<.05) with that reported for the period of wheel running activity in seven C×B RI strains (Schwartz, W. J., and Zimmerman, P., J. Neurosci.10:3685 1990).

KW - circadian rhythms

KW - genetics

KW - light

KW - locomotor activity

KW - quantitative trait loci

KW - Recombinant inbred mouse

UR - http://www.scopus.com/inward/record.url?scp=0028847289&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028847289&partnerID=8YFLogxK

U2 - 10.1007/BF02327578

DO - 10.1007/BF02327578

M3 - Article

C2 - 8540893

AN - SCOPUS:0028847289

VL - 25

SP - 545

EP - 556

JO - Behavior Genetics

JF - Behavior Genetics

SN - 0001-8244

IS - 6

ER -