Quiescence in 9L cells and correlation with radiosensitivity and PLD repair

Marc Mendonca, A. Rodriguez, E. L. Alpen

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The onset of quiescence, changes in X-ray sensitivity, and changes in capacity for potentially lethal damage (PLD) repair of unfed plateau-phase 9L44 cell cultures have been systemically investigated. The quiescent plateau phase in 9L cells was the result of nutrient deprivation and was not a cell contact effect. Eighty-five to 90% of the plateau-phase cells had a G1 DNA content and a growth fraction ≤0.15. The cell kinetic shifts in the population were temporally correlated with a developing radioresistance, which was characterized by a larger shoulder in the survival curve of the quiescent cells (D(q) = 5.71 Gy) versus exponentially growing cells (D(q) = 4.48 Gy). When the quiescent plateau-phase cells were refed, an increase in radiosensitivity resulted which approached that of exponentially growing 9L cells. Delayed plating experiments after irradiation of exponentially growing cells, quiescent plateau-phase cells, and synchronized early to mid-G1-phase cells indicated that while significant PLD repair was evident in all three populations, the quiescent 9L cells had a higher PLD repair capacity. Although data for immediate plating indicated that 9L cells may enter quiescence in the relatively radioresistant mid-G1 phase, the enhanced PLD repair capacity of quiescent cells cannot be explained by redistribution into G1 phase. When the unfed quiescent plateau-phase 9L cells were stimulated to reenter the cell cycle by replating into fresh medium, the first G1 was extended by 6 h compared with the G1 of exponentially growing or refed plateau-phase 9L cells. The relative radioresistance of the quiescent 9L cells may be the result of a longer potential repair time before reentry into the cell cycle.

Original languageEnglish (US)
Pages (from-to)433-447
Number of pages15
JournalRadiation Research
Volume117
Issue number3
StatePublished - 1989
Externally publishedYes

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Radiation Tolerance
radiation tolerance
lethal genes
damage
cells
plateaus
G1 Phase
interphase
radiation resistance
plating
cell cycle
Cell Cycle
deprivation
cycles
reentry
nutrients
shoulders

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Quiescence in 9L cells and correlation with radiosensitivity and PLD repair. / Mendonca, Marc; Rodriguez, A.; Alpen, E. L.

In: Radiation Research, Vol. 117, No. 3, 1989, p. 433-447.

Research output: Contribution to journalArticle

Mendonca, Marc ; Rodriguez, A. ; Alpen, E. L. / Quiescence in 9L cells and correlation with radiosensitivity and PLD repair. In: Radiation Research. 1989 ; Vol. 117, No. 3. pp. 433-447.
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