Rab10 regulates membrane transport through early endosomes of polarized Madin-Darby Canine Kidney cells

Clifford M. Babbey, Nahid Ahktar, Exing Wang, Carlos Chih Hsiung Chen, Barth D. Grant, Kenneth Dunn

Research output: Contribution to journalArticle

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Abstract

Rab10, a protein originally isolated from Madin-Darby Canine Kidney (MDCK) epithelial cells, belongs to a family of Rab proteins that includes Rab8 and Rab13. Although both Rab8 and Rab13 have been found to mediate polarized membrane transport, the function of Rab10 in mammalian cells has not yet been established. We have used quantitative confocal microscopy of polarized MDCK cells expressing GFP chimeras of wild-type and mutant forms of Rab10 to analyze the function of Rab10 in polarized cells. These studies demonstrate that Rab10 is specifically associated with the common endosomes of MDCK cells, accessible to endocytic probes internalized from either the apical or basolateral plasma membrane domains. Expression of mutant Rab10 defective for either GTP hydrolysis or GTP binding increased recycling from early compartments on the basolateral endocytic pathway without affecting recycling from later compartments or the apical recycling pathway. These results suggest that Rab10 mediates transport from basolateral sorting endosomes to common endosomes.

Original languageEnglish
Pages (from-to)3156-3175
Number of pages20
JournalMolecular Biology of the Cell
Volume17
Issue number7
DOIs
StatePublished - Jul 2006

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Madin Darby Canine Kidney Cells
Endosomes
Recycling
Guanosine Triphosphate
Membranes
Confocal Microscopy
Proteins
Hydrolysis
Epithelial Cells
Cell Membrane

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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Rab10 regulates membrane transport through early endosomes of polarized Madin-Darby Canine Kidney cells. / Babbey, Clifford M.; Ahktar, Nahid; Wang, Exing; Chen, Carlos Chih Hsiung; Grant, Barth D.; Dunn, Kenneth.

In: Molecular Biology of the Cell, Vol. 17, No. 7, 07.2006, p. 3156-3175.

Research output: Contribution to journalArticle

Babbey, Clifford M. ; Ahktar, Nahid ; Wang, Exing ; Chen, Carlos Chih Hsiung ; Grant, Barth D. ; Dunn, Kenneth. / Rab10 regulates membrane transport through early endosomes of polarized Madin-Darby Canine Kidney cells. In: Molecular Biology of the Cell. 2006 ; Vol. 17, No. 7. pp. 3156-3175.
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