Rabbit skeletal muscle glycogen synthase. II. Enzyme phosphorylation state and effector concentrations as interacting control parameters

Peter Roach, J. Larner

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56 Citations (Scopus)

Abstract

The effects of several inhibitors (ATP, ADP, AMP, UDP, and Pi) and activators (Mg2, glucose 6 P) of rabbit muscle glycogen synthase (UDP glucose:glycogen 4 α glucosyltranferase, EC 2.4.1.11) were studied in relation to the phosphorylation state of the purified enzyme. All the modifiers had increasing effects with enzyme of increasing alkali labile phosphate content. In experiments where combinations of effectors were present, it was apparent that (a) concentrations of modifiers in the physiological range could be significant in determining enzymic activity and (b) the sensitivity of the reaction rate to changes in phosphorylation state was critically dependent on the concentration of the small molecules. Changes in the phosphorylation of the enzyme corresponding to changes in the percent dephosphorylated activity reported in the literature for studies in vivo were capable of producing large alterations in glycogen synthase activity. Because the magnitudes of such changes were dependent on the effector concentrations, there may be an integration of local cellular control, through small molecule effects, with hormonal control, through the phosphorylation state of glycogen synthase.

Original languageEnglish (US)
Pages (from-to)1920-1925
Number of pages6
JournalJournal of Biological Chemistry
Volume251
Issue number7
StatePublished - 1976
Externally publishedYes

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Glycogen Synthase
Phosphorylation
Muscle
Skeletal Muscle
Rabbits
Enzymes
Uridine Diphosphate Glucose
Molecules
Uridine Diphosphate
Alkalies
Adenosine Monophosphate
Glycogen
Adenosine Diphosphate
Reaction rates
Adenosine Triphosphate
Phosphates
Glucose
Muscles
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

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abstract = "The effects of several inhibitors (ATP, ADP, AMP, UDP, and Pi) and activators (Mg2, glucose 6 P) of rabbit muscle glycogen synthase (UDP glucose:glycogen 4 α glucosyltranferase, EC 2.4.1.11) were studied in relation to the phosphorylation state of the purified enzyme. All the modifiers had increasing effects with enzyme of increasing alkali labile phosphate content. In experiments where combinations of effectors were present, it was apparent that (a) concentrations of modifiers in the physiological range could be significant in determining enzymic activity and (b) the sensitivity of the reaction rate to changes in phosphorylation state was critically dependent on the concentration of the small molecules. Changes in the phosphorylation of the enzyme corresponding to changes in the percent dephosphorylated activity reported in the literature for studies in vivo were capable of producing large alterations in glycogen synthase activity. Because the magnitudes of such changes were dependent on the effector concentrations, there may be an integration of local cellular control, through small molecule effects, with hormonal control, through the phosphorylation state of glycogen synthase.",
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AU - Larner, J.

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N2 - The effects of several inhibitors (ATP, ADP, AMP, UDP, and Pi) and activators (Mg2, glucose 6 P) of rabbit muscle glycogen synthase (UDP glucose:glycogen 4 α glucosyltranferase, EC 2.4.1.11) were studied in relation to the phosphorylation state of the purified enzyme. All the modifiers had increasing effects with enzyme of increasing alkali labile phosphate content. In experiments where combinations of effectors were present, it was apparent that (a) concentrations of modifiers in the physiological range could be significant in determining enzymic activity and (b) the sensitivity of the reaction rate to changes in phosphorylation state was critically dependent on the concentration of the small molecules. Changes in the phosphorylation of the enzyme corresponding to changes in the percent dephosphorylated activity reported in the literature for studies in vivo were capable of producing large alterations in glycogen synthase activity. Because the magnitudes of such changes were dependent on the effector concentrations, there may be an integration of local cellular control, through small molecule effects, with hormonal control, through the phosphorylation state of glycogen synthase.

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