Rac1, but not RhoA, signaling protects epithelial adherens jRacunction assembly during ATP depletion

Shobha Gopalakrishnan, Kenneth Dunn, James A. Marrs

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Rho family GTPase signaling regulates actin cytoskeleton and junctional complex assembly. Our previous work showed that RhoA signaling protects tight junctions from damage during ATP depletion. Here, we examined whether RhoA GTPase signaling protects adherens junction assembly during ATP depletion. Despite specific RhoA signaling- and ATP depletion-induced effects on adherens junction assembly, RhoA signaling did not alter adherens junction disassembly rates during ATP depletion. This shows that RhoA signaling specifically protects tight junctions from damage during ATP depletion. Rac1 GTPase signaling also regulates adherens junction assembly and therefore may regulate adherens junction assembly during ATP depletion. Indeed, we found that Rac1 signaling protects adherens junctions from damage during ATP depletion. Adherens junctions are regulated by various GTPases, including RhoA and Rac1, but adherens junctions are specifically protected by Rac1 signaling.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume283
Issue number1 52-1
StatePublished - 2002

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Adherens Junctions
Adenosine Triphosphate
GTP Phosphohydrolases
Tight Junctions
rho GTP-Binding Proteins
Actin Cytoskeleton
Actins

Keywords

  • Cadherin
  • Catenin
  • Ischemia

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Rac1, but not RhoA, signaling protects epithelial adherens jRacunction assembly during ATP depletion. / Gopalakrishnan, Shobha; Dunn, Kenneth; Marrs, James A.

In: American Journal of Physiology - Cell Physiology, Vol. 283, No. 1 52-1, 2002.

Research output: Contribution to journalArticle

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