Rac2, a hematopoiesis-specific Rho GTPase, specifically regulates mast cell protease gene expression in bone marrow-derived mast cells

Yi Gu, Michael C. Byrne, Nivanka C. Paranavitana, Bruce Aronow, Jamie E. Siefring, Maria D'Souza, Heidi F. Horton, Lawrence Quilliam, David A. Williams

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Rho family GTPases activate intracellular kinase cascades to modulate transcription of multiple genes. Previous studies have examined the roles of the ubiquitously expressed Rho GTPase, Rac1, in regulation of gene expression in cell lines and implicated NF-κB, serum response factor, and kinase signaling pathways in this regulation. To understand the role of the closely related but hematopoiesis-specific Rho GTPase, Rac2, in regulation of gene transcription, we compared the gene expression profiles between wild-type and Rac2-/- bone marrow-derived mast cells. Our data demonstrate remarkable specificity in the regulation of gene expression by Rac2 versus Rac1. Microarray analysis demonstrated that expression of 38 known genes was significantly altered in Rac2-/- mast cells after cytokine stimulation compared with those in wild-type cells. Of these, the expression of the mouse mast cell protease 7 (MMCP-7) gene in wild-type cells was highly induced at the transcriptional level after stimulation with stem cell factor (SCF). In spite of compensatorily increased expression of Rac1 in Rac2-deficient cells, SCF-induced MMCP-7 transcription did not occur. Surprisingly, the loss of MMCP-7 induction was not due to decreased activation of NF-κB, a transcription factor postulated to lie downstream of Rac1 and known to play a critical role in hematopoietic cell differentiation and proliferation. However, the activities of c-Jun N-terminal kinases (JNKs) were markedly decreased in Rac2-/- mast cells. Our results suggest that cytokine-stimulated activation of MMCP-7 gene transcription is selectively regulated by a Rac2-dependent JNK signaling pathway in primary mast cells and imply a remarkable specificity in the regulation of transcriptional activity by these two highly related Rho GTPases.

Original languageEnglish (US)
Pages (from-to)7645-7657
Number of pages13
JournalMolecular and Cellular Biology
Volume22
Issue number21
DOIs
StatePublished - Nov 2002
Externally publishedYes

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rho GTP-Binding Proteins
Hematopoiesis
Mast Cells
Peptide Hydrolases
Bone Marrow
Gene Expression
Stem Cell Factor
Genes
Gene Expression Regulation
Phosphotransferases
Cytokines
JNK Mitogen-Activated Protein Kinases
Microarray Analysis
Transcriptome
Cell Differentiation
Transcription Factors
Cell Proliferation
Cell Line
Mouse Tpsab1 protein

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Rac2, a hematopoiesis-specific Rho GTPase, specifically regulates mast cell protease gene expression in bone marrow-derived mast cells. / Gu, Yi; Byrne, Michael C.; Paranavitana, Nivanka C.; Aronow, Bruce; Siefring, Jamie E.; D'Souza, Maria; Horton, Heidi F.; Quilliam, Lawrence; Williams, David A.

In: Molecular and Cellular Biology, Vol. 22, No. 21, 11.2002, p. 7645-7657.

Research output: Contribution to journalArticle

Gu, Yi ; Byrne, Michael C. ; Paranavitana, Nivanka C. ; Aronow, Bruce ; Siefring, Jamie E. ; D'Souza, Maria ; Horton, Heidi F. ; Quilliam, Lawrence ; Williams, David A. / Rac2, a hematopoiesis-specific Rho GTPase, specifically regulates mast cell protease gene expression in bone marrow-derived mast cells. In: Molecular and Cellular Biology. 2002 ; Vol. 22, No. 21. pp. 7645-7657.
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AU - Byrne, Michael C.

AU - Paranavitana, Nivanka C.

AU - Aronow, Bruce

AU - Siefring, Jamie E.

AU - D'Souza, Maria

AU - Horton, Heidi F.

AU - Quilliam, Lawrence

AU - Williams, David A.

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