Radiation pneumonitis in mice: A severe injury model for pneumocyte engraftment from bone marrow

Neil D. Theise, Octavian Henegariu, Joanna Grove, Jayishree Jagirdar, Peter N. Kao, James M. Crawford, Sunil Badve, Romil Saxena, Diane S. Krause

Research output: Contribution to journalArticle

174 Citations (Scopus)

Abstract

Objective. To better understand the process by which pneumocytes can be derived from bone marrow cells, we investigated the in vivo kinetics of such engraftment following lethal irradiation. Methods. A cohort of lethally irradiated B6D2F1 female mice received whole bone marrow transplants (BMT) from age-matched male donors and were sacrificed at days 1, 3, 5, and 7 and months 2, 4, and 6 post-BMT (n = 3 for each time point). Additionally, 2 female mice who had received 200 male fluorescence-activated cell sorter (FACS)-sorted CD34+lin- cells were sacrificed 8 months post-BMT. Results. Lethal irradiation caused histologic evidence of pneumonitis including alveolar breakdown and hemorrhage beginning at day 3. To identify male-derived pneumocytes, simultaneous fluorescence in situ hybridization (FISH) for Y-chromosome and surfactant B messenger RNA was performed on lung tissue. Y+ type II pneumocytes were engrafted as early as day 5 posttransplant, and eventually from 2 to 14% of the pneumocytes were donor derived in individual mice. Co-staining for epithelial-specific cytokeratins demonstrated that by 2 months, marrow-derived pneumocytes could comprise entire alveoli, suggesting that type I cells derived from type II pneumocytes. Conclusion. We conclude that alveolar lining cells derive from bone marrow cells immediately after acute injury. Also, the CD34+lin- subpopulation is capable of such pulmonary engraftment.

Original languageEnglish (US)
Pages (from-to)1333-1338
Number of pages6
JournalExperimental Hematology
Volume30
Issue number11
DOIs
StatePublished - Nov 1 2002
Externally publishedYes

Fingerprint

Radiation Pneumonitis
Alveolar Epithelial Cells
Bone Marrow
Wounds and Injuries
Transplants
Bone Marrow Cells
Keratin-2
Chromosomes, Human, 4-5
Lung
Y Chromosome
Fluorescence In Situ Hybridization
Surface-Active Agents
Pneumonia
Fluorescence
Staining and Labeling
Hemorrhage
Messenger RNA

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Theise, N. D., Henegariu, O., Grove, J., Jagirdar, J., Kao, P. N., Crawford, J. M., ... Krause, D. S. (2002). Radiation pneumonitis in mice: A severe injury model for pneumocyte engraftment from bone marrow. Experimental Hematology, 30(11), 1333-1338. https://doi.org/10.1016/S0301-472X(02)00931-1

Radiation pneumonitis in mice : A severe injury model for pneumocyte engraftment from bone marrow. / Theise, Neil D.; Henegariu, Octavian; Grove, Joanna; Jagirdar, Jayishree; Kao, Peter N.; Crawford, James M.; Badve, Sunil; Saxena, Romil; Krause, Diane S.

In: Experimental Hematology, Vol. 30, No. 11, 01.11.2002, p. 1333-1338.

Research output: Contribution to journalArticle

Theise, ND, Henegariu, O, Grove, J, Jagirdar, J, Kao, PN, Crawford, JM, Badve, S, Saxena, R & Krause, DS 2002, 'Radiation pneumonitis in mice: A severe injury model for pneumocyte engraftment from bone marrow', Experimental Hematology, vol. 30, no. 11, pp. 1333-1338. https://doi.org/10.1016/S0301-472X(02)00931-1
Theise, Neil D. ; Henegariu, Octavian ; Grove, Joanna ; Jagirdar, Jayishree ; Kao, Peter N. ; Crawford, James M. ; Badve, Sunil ; Saxena, Romil ; Krause, Diane S. / Radiation pneumonitis in mice : A severe injury model for pneumocyte engraftment from bone marrow. In: Experimental Hematology. 2002 ; Vol. 30, No. 11. pp. 1333-1338.
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