Radioactive in situ hybridization to detect gene expression in skeletal Tissue Sections

Kyu Sang Joeng, Jenna Regan, Fanxin Long

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In situ hybridization (ISH) using RNA probes is a valuable technique to characterize gene expression patterns in animal tissues. It provides valuable spatial information about gene expression. Compared to the nonradioactive alternatives,35S radioactive ISH generally provides higher sensitivity. Here, we describe the procedure for35S ISH on paraffin sections from the skeletal tissues of mouse embryos.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages217-232
Number of pages16
Volume1130
DOIs
StatePublished - 2014
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1130
ISSN (Print)10643745

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Joeng, K. S., Regan, J., & Long, F. (2014). Radioactive in situ hybridization to detect gene expression in skeletal Tissue Sections. In Methods in Molecular Biology (Vol. 1130, pp. 217-232). (Methods in Molecular Biology; Vol. 1130). https://doi.org/10.1007/978-1-62703-989-5-16