In situ hybridization (ISH) using RNA probes is a valuable technique to characterize gene expression patterns in animal tissues. It provides valuable spatial information about gene expression. Compared to the nonradioactive alternatives,35S radioactive ISH generally provides higher sensitivity. Here, we describe the procedure for35S ISH on paraffin sections from the skeletal tissues of mouse embryos.
|Original language||English (US)|
|Title of host publication||Methods in Molecular Biology|
|Number of pages||16|
|State||Published - 2014|
|Name||Methods in Molecular Biology|
ASJC Scopus subject areas
- Molecular Biology