Radioactive in situ hybridization to detect gene expression in skeletal Tissue Sections

Kyu Sang Joeng, Jenna Regan, Fanxin Long

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

In situ hybridization (ISH) using RNA probes is a valuable technique to characterize gene expression patterns in animal tissues. It provides valuable spatial information about gene expression. Compared to the nonradioactive alternatives,35S radioactive ISH generally provides higher sensitivity. Here, we describe the procedure for35S ISH on paraffin sections from the skeletal tissues of mouse embryos.

Original languageEnglish (US)
Title of host publicationSkeletal Development and Repair
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages217-232
Number of pages16
ISBN (Print)9781627039888
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1130
ISSN (Print)1064-3745

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Keywords

  • Bone
  • Embryos
  • In situ hybridization
  • Mouse
  • Paraffin-embedded sections
  • Radioactive
  • Riboprobe

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Joeng, K. S., Regan, J., & Long, F. (2014). Radioactive in situ hybridization to detect gene expression in skeletal Tissue Sections. In Skeletal Development and Repair: Methods and Protocols (pp. 217-232). (Methods in Molecular Biology; Vol. 1130). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-989-5_16