Raman spectroscopy of HIV-1 antigen and antibody

Pavel V. Zinin, Ningjie Hu, Lori E. Kamemoto, Qigui Yu, Anupam K. Misra, Shiv K. Sharma

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

Raman spectra of anti-HIV-1 antibody, HIV-1 antigen (p24), and HIV-1 antibody-antigen complex have been measured in near-infrared and UV regions: 785 nm; 830 nm; and 244 nm laser excitations. The spectrum of the HIV-1 antigen was excited with an infrared laser and contains numerous Raman peaks. The most prominent peaks are broad bands at 1343, 1449, 1609 and 1655 cm-1, which are characteristic of the Raman spectra of biological cells. The UV Raman spectrum of the HIV-1 antigen has a completely different structure. It has two strong peaks at 1613 cm-1 and 1173 cm-1. The peak at 1613 cm-1 is associated with vibrations of the aromatic amino acids tyrosine (Tyr) and tryptophan (Try). The second strongest peak at 1173 cm -1 is associated with the vibration of Tyr. The Raman peak pattern of the HIV-1 antigen-antibody complex is very similar to that of the HIV-1 antigen. The only difference is that the peak at 1007 cm-1 of the Raman spectrum of the HIV-1 antigen-antibody complex is slightly enhanced compared to that of the HIV-1 antigen. This indicates that the peaks of the HIV-1 antigen dominate the Raman spectrum of the HIV-1 antigen-antibody complex.

Original languageEnglish (US)
Title of host publicationSmart Biomedical and Physiological Sensor Technology VIII
DOIs
StatePublished - Sep 26 2011
EventSmart Biomedical and Physiological Sensor Technology VIII - Orlando, FL, United States
Duration: Apr 28 2011Apr 28 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8025
ISSN (Print)1605-7422

Other

OtherSmart Biomedical and Physiological Sensor Technology VIII
CountryUnited States
CityOrlando, FL
Period4/28/114/28/11

Keywords

  • HIV-1 antibody
  • HIV-1 p24 antigen
  • IL-17A antigen
  • micro-Raman
  • monocytes
  • UV Raman

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Zinin, P. V., Hu, N., Kamemoto, L. E., Yu, Q., Misra, A. K., & Sharma, S. K. (2011). Raman spectroscopy of HIV-1 antigen and antibody. In Smart Biomedical and Physiological Sensor Technology VIII [80250D] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8025). https://doi.org/10.1117/12.887169