Ran stimulates spindle assembly by altering microtubule dynamics and the balance of motor activities

Andrew Wilde, Sofia B. Lizarraga, Lijun Zhang, Christiane Wiese, Neal R. Gliksman, Claire E. Walczak, Yixian Zheng

Research output: Contribution to journalArticle

176 Scopus citations

Abstract

The guanosine tri-phosphatase Ran stimulates assembly of microtubule spindles. However, it is not known what aspects of the microtubule cytoskeleton are subject to regulation by Ran in mitosis. Here we show that Ran-GTP stimulates microtubule assembly by increasing the rescue frequency of microtubules three- to eightfold. In addition to changing microtubule dynamics, Ran-GTP also alters the balance of motor activities, partly as a result of an increase in the amount of motile Eg5, a plus-end-directed microtubule motor that is essential for spindle formation. Thus, Ran regulates multiple processes that are involved in spindle assembly.

Original languageEnglish (US)
Pages (from-to)221-227
Number of pages7
JournalNature Cell Biology
Volume3
Issue number3
DOIs
StatePublished - Mar 20 2001

ASJC Scopus subject areas

  • Cell Biology

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