Rapid, accurate and sensitive determination of molecular weight of 15N and 13C labeled recombinant proteins by electrospray ionization mass spectrometry

Timothy G. Heath, Venkataraman Thanabal, Qizhuang Ye

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Electrospray ionization mass spectrometry (ES-MS) was utilized to accurately measure the molecular weight of uniformly 15N and 13C/15N isotope labeled stromelysin catalytic domain (20 kDa), which in turn, was used to determine the degree of isotope enrichment in the protein.

Original languageEnglish (US)
Pages (from-to)367-372
Number of pages6
JournalBiotechnology Techniques
Volume7
Issue number5
DOIs
StatePublished - May 1993
Externally publishedYes

Fingerprint

Recombinant proteins
Electrospray ionization
Electrospray Ionization Mass Spectrometry
Recombinant Proteins
Isotopes
Mass spectrometry
Molecular Weight
Molecular weight
Matrix Metalloproteinase 3
Catalytic Domain
Proteins

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biochemistry

Cite this

Rapid, accurate and sensitive determination of molecular weight of 15N and 13C labeled recombinant proteins by electrospray ionization mass spectrometry. / Heath, Timothy G.; Thanabal, Venkataraman; Ye, Qizhuang.

In: Biotechnology Techniques, Vol. 7, No. 5, 05.1993, p. 367-372.

Research output: Contribution to journalArticle

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