Rapid analysis of lymphocyte subsets in cord blood

P. K. Kotylo, J. C. Baenzinger, M. C. Yoder, W. A. Engle, C. D. Bolinger

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Several investigators have enumerated cellular populations in neonatal cord blood with variable results. In this study, the authors established reference ranges for lymphocyte subsets in cord blood from healthy newborns using a whole blood lysis technique on the Coulter® Immunoprep Epics Leukocyte Preparation System (Coulter Immunology, Hialeah, FL). All analyses were performed on a flow cytometer by gating on forward angle versus 90° light scatter. Lymphocytes demonstrated all surface markers examined, including T4, T8, T3, T11, B1, NKH-1, I3, and 4B4; 2H4 suppressor inducer lymphocytes were prominent in neonatal blood. The authors think this standardized system may be suitable for use in neonatal and pediatric patients because it quickly processes small aliquots of whole blood with minimal sample manipulation.

Original languageEnglish (US)
Pages (from-to)263-266
Number of pages4
JournalAmerican journal of clinical pathology
Volume93
Issue number2
DOIs
StatePublished - Jan 1 1990

Keywords

  • cord blood
  • lymphocyte subsets
  • monoclonal antibodies
  • whole blood lysis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Kotylo, P. K., Baenzinger, J. C., Yoder, M. C., Engle, W. A., & Bolinger, C. D. (1990). Rapid analysis of lymphocyte subsets in cord blood. American journal of clinical pathology, 93(2), 263-266. https://doi.org/10.1093/ajcp/93.2.263