Rapid analysis of lymphocyte subsets in cord blood

P. K. Kotylo, J. C. Baenzinger, Mervin Yoder, W. A. Engle, C. D. Bolinger

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Several investigators have enumerated cellular populations in neonatal cord blood with variable results. In this study, the authors established reference ranges for lymphocyte subsets in cord blood from healthy newborns using a whole blood lysis technique on the Coulter® Immunoprep Epics Leukocyte Preparation System (Coulter Immunology, Hialeah, FL). All analyses were performed on a flow cytometer by gating on forward angle versus 90° light scatter. Lymphocytes demonstrated all surface markers examined, including T4, T8, T3, T11, B1, NKH-1, I3, and 4B4; 2H4 suppressor inducer lymphocytes were prominent in neonatal blood. The authors think this standardized system may be suitable for use in neonatal and pediatric patients because it quickly processes small aliquots of whole blood with minimal sample manipulation.

Original languageEnglish
Pages (from-to)263-266
Number of pages4
JournalAmerican Journal of Clinical Pathology
Volume93
Issue number2
StatePublished - 1990

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Lymphocyte Subsets
Fetal Blood
Lymphocytes
Allergy and Immunology
Reference Values
Leukocytes
Research Personnel
Newborn Infant
Pediatrics
Light
Population

Keywords

  • cord blood
  • lymphocyte subsets
  • monoclonal antibodies
  • whole blood lysis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Kotylo, P. K., Baenzinger, J. C., Yoder, M., Engle, W. A., & Bolinger, C. D. (1990). Rapid analysis of lymphocyte subsets in cord blood. American Journal of Clinical Pathology, 93(2), 263-266.

Rapid analysis of lymphocyte subsets in cord blood. / Kotylo, P. K.; Baenzinger, J. C.; Yoder, Mervin; Engle, W. A.; Bolinger, C. D.

In: American Journal of Clinical Pathology, Vol. 93, No. 2, 1990, p. 263-266.

Research output: Contribution to journalArticle

Kotylo, PK, Baenzinger, JC, Yoder, M, Engle, WA & Bolinger, CD 1990, 'Rapid analysis of lymphocyte subsets in cord blood', American Journal of Clinical Pathology, vol. 93, no. 2, pp. 263-266.
Kotylo PK, Baenzinger JC, Yoder M, Engle WA, Bolinger CD. Rapid analysis of lymphocyte subsets in cord blood. American Journal of Clinical Pathology. 1990;93(2):263-266.
Kotylo, P. K. ; Baenzinger, J. C. ; Yoder, Mervin ; Engle, W. A. ; Bolinger, C. D. / Rapid analysis of lymphocyte subsets in cord blood. In: American Journal of Clinical Pathology. 1990 ; Vol. 93, No. 2. pp. 263-266.
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