Rapid and efficient separation and identification of the two molecular forms of human adenosine deaminase by thin-layer gel filtration chromatography

Pang Fai Ma, Steve Betras, Gary Dunnington

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Two molecular forms of adenosine deaminase have been found in human tissues. The column gel filtration method has been used for the separation of the two enzyme forms. Routine separation and analysis of the enzyme forms based on the molecular size difference can be achieved by thin-layer gel filtration on Sephadex G-200 superfine gel. The thin-layer method has been found to be more rapid and efficient than the column method. Enzymes in crude preparations can be studied effectively with the thin-layer method.

Original languageEnglish (US)
Pages (from-to)177-182
Number of pages6
JournalAnalytical biochemistry
Volume75
Issue number1
DOIs
StatePublished - Sep 1976
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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