Rapid Communication: Adrenalectomy Attenuates Stress‐Induced Elevations in Extracellular Glutamate Concentrations in the Hippocampus

Martin T. Lowy, Laura Gault, Bryan Yamamoto

Research output: Contribution to journalArticle

302 Citations (Scopus)

Abstract

Abstract— Glucocorticoids and stress have deleterious effects on hippocampal cell morphology and survival. It has been hypothesized that these effects are mediated via an excitatory amino acid mechanism. The present study was designed to evaluate the effects of acute stress on the extracellular levels of glutamate in the hippocampus and to determine if adrenalectomy modifies this response. Rats were adrenalectomized or sham‐adrenalectomized and implanted with microdialysis probes in the CAS region of the hippocampus. Three days later rats were subjected to an acute 1 ‐h period of immobilization stress. Stress significantly increased extracellular glutamate levels in the sham‐operated rats, which peaked at 20 min following the initiation of stress. Extracellular glutamate levels also increased immediately following the termination of stress. In the adrenalectomized rats there was a 30% decrease in basal extracellular concentrations of glutamate and a marked attenuation (‐70%) of the stress‐induced increase in extracellular glutamate levels. Extracellular concentrations of taurine were not modified by adrenalectomy and did not change in response to stress. These results suggest that glucocorticoid‐in‐duced elevations in extracellular glutamate concentrations may contribute to the deleterious effects of stress on hippocampal neurons.

Original languageEnglish (US)
Pages (from-to)1957-1960
Number of pages4
JournalJournal of Neurochemistry
Volume61
Issue number5
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Adrenalectomy
Glutamic Acid
Hippocampus
Communication
Rats
Excitatory Amino Acids
Taurine
Microdialysis
Immobilization
Glucocorticoids
Cell Survival
Neurons

Keywords

  • aurine
  • drenalectom
  • eurotoxioit
  • lucocorticoid
  • lutamat
  • Stres
  • xcitatory amino acid

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Rapid Communication : Adrenalectomy Attenuates Stress‐Induced Elevations in Extracellular Glutamate Concentrations in the Hippocampus. / Lowy, Martin T.; Gault, Laura; Yamamoto, Bryan.

In: Journal of Neurochemistry, Vol. 61, No. 5, 1993, p. 1957-1960.

Research output: Contribution to journalArticle

@article{9a3fea34e5b74e64b884aaab0b4e96f8,
title = "Rapid Communication: Adrenalectomy Attenuates Stress‐Induced Elevations in Extracellular Glutamate Concentrations in the Hippocampus",
abstract = "Abstract— Glucocorticoids and stress have deleterious effects on hippocampal cell morphology and survival. It has been hypothesized that these effects are mediated via an excitatory amino acid mechanism. The present study was designed to evaluate the effects of acute stress on the extracellular levels of glutamate in the hippocampus and to determine if adrenalectomy modifies this response. Rats were adrenalectomized or sham‐adrenalectomized and implanted with microdialysis probes in the CAS region of the hippocampus. Three days later rats were subjected to an acute 1 ‐h period of immobilization stress. Stress significantly increased extracellular glutamate levels in the sham‐operated rats, which peaked at 20 min following the initiation of stress. Extracellular glutamate levels also increased immediately following the termination of stress. In the adrenalectomized rats there was a 30{\%} decrease in basal extracellular concentrations of glutamate and a marked attenuation (‐70{\%}) of the stress‐induced increase in extracellular glutamate levels. Extracellular concentrations of taurine were not modified by adrenalectomy and did not change in response to stress. These results suggest that glucocorticoid‐in‐duced elevations in extracellular glutamate concentrations may contribute to the deleterious effects of stress on hippocampal neurons.",
keywords = "aurine, drenalectom, eurotoxioit, lucocorticoid, lutamat, Stres, xcitatory amino acid",
author = "Lowy, {Martin T.} and Laura Gault and Bryan Yamamoto",
year = "1993",
doi = "10.1111/j.1471-4159.1993.tb09839.x",
language = "English (US)",
volume = "61",
pages = "1957--1960",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Rapid Communication

T2 - Adrenalectomy Attenuates Stress‐Induced Elevations in Extracellular Glutamate Concentrations in the Hippocampus

AU - Lowy, Martin T.

AU - Gault, Laura

AU - Yamamoto, Bryan

PY - 1993

Y1 - 1993

N2 - Abstract— Glucocorticoids and stress have deleterious effects on hippocampal cell morphology and survival. It has been hypothesized that these effects are mediated via an excitatory amino acid mechanism. The present study was designed to evaluate the effects of acute stress on the extracellular levels of glutamate in the hippocampus and to determine if adrenalectomy modifies this response. Rats were adrenalectomized or sham‐adrenalectomized and implanted with microdialysis probes in the CAS region of the hippocampus. Three days later rats were subjected to an acute 1 ‐h period of immobilization stress. Stress significantly increased extracellular glutamate levels in the sham‐operated rats, which peaked at 20 min following the initiation of stress. Extracellular glutamate levels also increased immediately following the termination of stress. In the adrenalectomized rats there was a 30% decrease in basal extracellular concentrations of glutamate and a marked attenuation (‐70%) of the stress‐induced increase in extracellular glutamate levels. Extracellular concentrations of taurine were not modified by adrenalectomy and did not change in response to stress. These results suggest that glucocorticoid‐in‐duced elevations in extracellular glutamate concentrations may contribute to the deleterious effects of stress on hippocampal neurons.

AB - Abstract— Glucocorticoids and stress have deleterious effects on hippocampal cell morphology and survival. It has been hypothesized that these effects are mediated via an excitatory amino acid mechanism. The present study was designed to evaluate the effects of acute stress on the extracellular levels of glutamate in the hippocampus and to determine if adrenalectomy modifies this response. Rats were adrenalectomized or sham‐adrenalectomized and implanted with microdialysis probes in the CAS region of the hippocampus. Three days later rats were subjected to an acute 1 ‐h period of immobilization stress. Stress significantly increased extracellular glutamate levels in the sham‐operated rats, which peaked at 20 min following the initiation of stress. Extracellular glutamate levels also increased immediately following the termination of stress. In the adrenalectomized rats there was a 30% decrease in basal extracellular concentrations of glutamate and a marked attenuation (‐70%) of the stress‐induced increase in extracellular glutamate levels. Extracellular concentrations of taurine were not modified by adrenalectomy and did not change in response to stress. These results suggest that glucocorticoid‐in‐duced elevations in extracellular glutamate concentrations may contribute to the deleterious effects of stress on hippocampal neurons.

KW - aurine

KW - drenalectom

KW - eurotoxioit

KW - lucocorticoid

KW - lutamat

KW - Stres

KW - xcitatory amino acid

UR - http://www.scopus.com/inward/record.url?scp=0027378117&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027378117&partnerID=8YFLogxK

U2 - 10.1111/j.1471-4159.1993.tb09839.x

DO - 10.1111/j.1471-4159.1993.tb09839.x

M3 - Article

C2 - 7901339

AN - SCOPUS:0027378117

VL - 61

SP - 1957

EP - 1960

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 5

ER -