Rapid detection of Enterococcus spp. direct from blood culture bottles using Enterococcus QuickFISH Method

A multicenter investigation

Melissa K. Deck, Erica S. Anderson, Rebecca J. Buckner, Georgia Colasante, Thomas Davis, James M. Coull, Benjamin Crystal, Phyllis Della Latta, Martin Fuchs, Deanna Fuller, Will Harris, Kevin Hazen, Lisa L. Klimas, Daniel Lindao, Michelle C. Meltzer, Margie Morgan, Janeen Shepard, Sharon Stevens, Fann Wu, Mark J. Fiandaca

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The performance of a diagnostic method for detection and identification of Enterococcus spp. directly from positive blood culture was evaluated in a clinical study. The method, Enterococcus QuickFISH BC, is a second-generation peptide nucleic acid (PNA) fluorescence in situ hybridization (FISH) test, which uses a simplified, faster assay procedure. The test uses fluorescently labeled PNA probes targeting 16S rRNA to differentiate Enterococcus faecalis from other Enterococcus spp. by the color of the cellular fluorescence. Three hundred fifty-six routine blood culture samples were tested; only 2 discordant results were recorded. The sensitivities for detection of Enterococcus faecalis and non- faecalis Enterococcus were 100% (106/106) and 97.0% (65/67), respectively, and the combined specificity of the assay was 100%. The combined positive and negative predictive values of the assay were 100% (171/171) and 98.9% (185/187), respectively.

Original languageEnglish
Pages (from-to)338-342
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume78
Issue number4
DOIs
StatePublished - 2014

Fingerprint

Enterococcus faecalis
Enterococcus
Peptide Nucleic Acids
Nucleic Acid Probes
Fluorescence In Situ Hybridization
Color
Fluorescence
Blood Culture

Keywords

  • Enterococcus
  • PNA
  • QuickFISH
  • Septicemia

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Rapid detection of Enterococcus spp. direct from blood culture bottles using Enterococcus QuickFISH Method : A multicenter investigation. / Deck, Melissa K.; Anderson, Erica S.; Buckner, Rebecca J.; Colasante, Georgia; Davis, Thomas; Coull, James M.; Crystal, Benjamin; Latta, Phyllis Della; Fuchs, Martin; Fuller, Deanna; Harris, Will; Hazen, Kevin; Klimas, Lisa L.; Lindao, Daniel; Meltzer, Michelle C.; Morgan, Margie; Shepard, Janeen; Stevens, Sharon; Wu, Fann; Fiandaca, Mark J.

In: Diagnostic Microbiology and Infectious Disease, Vol. 78, No. 4, 2014, p. 338-342.

Research output: Contribution to journalArticle

Deck, MK, Anderson, ES, Buckner, RJ, Colasante, G, Davis, T, Coull, JM, Crystal, B, Latta, PD, Fuchs, M, Fuller, D, Harris, W, Hazen, K, Klimas, LL, Lindao, D, Meltzer, MC, Morgan, M, Shepard, J, Stevens, S, Wu, F & Fiandaca, MJ 2014, 'Rapid detection of Enterococcus spp. direct from blood culture bottles using Enterococcus QuickFISH Method: A multicenter investigation', Diagnostic Microbiology and Infectious Disease, vol. 78, no. 4, pp. 338-342. https://doi.org/10.1016/j.diagmicrobio.2013.12.004
Deck, Melissa K. ; Anderson, Erica S. ; Buckner, Rebecca J. ; Colasante, Georgia ; Davis, Thomas ; Coull, James M. ; Crystal, Benjamin ; Latta, Phyllis Della ; Fuchs, Martin ; Fuller, Deanna ; Harris, Will ; Hazen, Kevin ; Klimas, Lisa L. ; Lindao, Daniel ; Meltzer, Michelle C. ; Morgan, Margie ; Shepard, Janeen ; Stevens, Sharon ; Wu, Fann ; Fiandaca, Mark J. / Rapid detection of Enterococcus spp. direct from blood culture bottles using Enterococcus QuickFISH Method : A multicenter investigation. In: Diagnostic Microbiology and Infectious Disease. 2014 ; Vol. 78, No. 4. pp. 338-342.
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