Rapid determination of advanced glycation end products of proteins using MALDI-TOF-MS and PERL script peptide searching algorithm

Yinong Zhang, Ross R. Cocklin, Keshore R. Bidasee, Mu Wang

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Advanced glycation end products (AGEs), which are composed of various glucose or carbohydrate adducts, are thought to be responsible for several diabetic and age-related complications. However, to date, specific sites on proteins that are modified by AGEs remain largely unknown. We report here the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to determine the type and localization of several AGEs formed in vitro on human beta-2-microglobulin (β 2M),and in vivo on type 2 ryanodine receptor calcium-release channel (RyR2), and sarco(endo)plasmic reticulum (SERCA2a). A PERL script algorithm, developed in-house, makes searching the relatively large amount of data generated by the MALDI-MS more manageable.The outstanding sensitivity of MALDI-TOF-MS coupled with the PERL script algorithm allows such an approach to be a very useful tool in detecting AGEs and other post-translational modifications. We believe that this method could be an important tool when searching for post-translational modifications on proteins.

Original languageEnglish (US)
Pages (from-to)224-230
Number of pages7
JournalJournal of Biomolecular Techniques
Volume14
Issue number3
StatePublished - Sep 1 2003

Keywords

  • Advanced-glycation end products
  • Mass spectrometry
  • Matrix-assisted laser desorption/ionization
  • Post-translational modification

ASJC Scopus subject areas

  • Molecular Biology

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